Determination of the specific activity of sheep plasma amino acids using high-performance liquid chromatography: Comparison study between liquid scintillation counter and on-line flow-through detector

Lasker S. Ahmed, Helen Moorehead, Catherine A. Leitch, Edward A. Liechty

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A method was developed for the determination of the specific activities of leucine and phenylalanine in plasma using a flow-through scintillation counter coupled with high-performance liquid chromatography components. Results were compared with those obtained from liquid scintillation counting. Differences in the specific activities of leucine and phenylalanine between the two methods were not statistically significant. We concluded that flow- through radioactivity detection can be used for quantitative amino acid assays. However, the minimum activity that can be detected may be prohibitively low in certain applications.

Original languageEnglish
Pages (from-to)27-35
Number of pages9
JournalJournal of Chromatography B: Biomedical Applications
Volume710
Issue number1-2
DOIs
StatePublished - Jun 12 1998

Fingerprint

Scintillation counters
High performance liquid chromatography
Phenylalanine
Leucine
Detectors
Plasmas
Amino Acids
Radioactivity
Liquids
Scintillation
Assays

Keywords

  • Leucine
  • Phenylalanine

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Determination of the specific activity of sheep plasma amino acids using high-performance liquid chromatography : Comparison study between liquid scintillation counter and on-line flow-through detector. / Ahmed, Lasker S.; Moorehead, Helen; Leitch, Catherine A.; Liechty, Edward A.

In: Journal of Chromatography B: Biomedical Applications, Vol. 710, No. 1-2, 12.06.1998, p. 27-35.

Research output: Contribution to journalArticle

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