Determination of trace isoflavone phytoestrogens in biological materials by capillary electrochromatograph

Jason A. Starkey, Yehia Mechref, Chang Kyu Byun, Rosemary Steinmetz, John Fuqua, Ora H. Pescovitz, Milos V. Novotny

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Capillary electrochromatography using a specialty monolithic matrix was utilized in developing a rapid and highly efficient separation of isoflavones in biological materials. Without a preconcentration technique, it is relatively easy to reach ppm-ppb concentrations of these compounds in soy-based foods and verify them structurally using a photodiode array detector. With on-column preconcentration, we were able to measure low-ppb levels in human serum. Using blood samples from human volunteers, whose diet was supplemented by a soy-based product, the method has been validated for high-throughput screening of isoflavones in clinical studies.

Original languageEnglish
Pages (from-to)5998-6005
Number of pages8
JournalAnalytical Chemistry
Volume74
Issue number23
DOIs
StatePublished - Dec 1 2002

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Phytoestrogens
Isoflavones
Biological materials
Nutrition
Photodiodes
Screening
Blood
Throughput
Detectors

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Starkey, J. A., Mechref, Y., Byun, C. K., Steinmetz, R., Fuqua, J., Pescovitz, O. H., & Novotny, M. V. (2002). Determination of trace isoflavone phytoestrogens in biological materials by capillary electrochromatograph. Analytical Chemistry, 74(23), 5998-6005. https://doi.org/10.1021/ac025929b

Determination of trace isoflavone phytoestrogens in biological materials by capillary electrochromatograph. / Starkey, Jason A.; Mechref, Yehia; Byun, Chang Kyu; Steinmetz, Rosemary; Fuqua, John; Pescovitz, Ora H.; Novotny, Milos V.

In: Analytical Chemistry, Vol. 74, No. 23, 01.12.2002, p. 5998-6005.

Research output: Contribution to journalArticle

Starkey, JA, Mechref, Y, Byun, CK, Steinmetz, R, Fuqua, J, Pescovitz, OH & Novotny, MV 2002, 'Determination of trace isoflavone phytoestrogens in biological materials by capillary electrochromatograph', Analytical Chemistry, vol. 74, no. 23, pp. 5998-6005. https://doi.org/10.1021/ac025929b
Starkey, Jason A. ; Mechref, Yehia ; Byun, Chang Kyu ; Steinmetz, Rosemary ; Fuqua, John ; Pescovitz, Ora H. ; Novotny, Milos V. / Determination of trace isoflavone phytoestrogens in biological materials by capillary electrochromatograph. In: Analytical Chemistry. 2002 ; Vol. 74, No. 23. pp. 5998-6005.
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