Determination of trace isoflavone phytoestrogens in biological materials by capillary electrochromatograph

Jason A. Starkey, Yehia Mechref, Chang Kyu Byun, Rosemary Steinmetz, John S. Fuqua, Ora H. Pescovitz, Milos V. Novotny

Research output: Contribution to journalArticlepeer-review

40 Scopus citations


Capillary electrochromatography using a specialty monolithic matrix was utilized in developing a rapid and highly efficient separation of isoflavones in biological materials. Without a preconcentration technique, it is relatively easy to reach ppm-ppb concentrations of these compounds in soy-based foods and verify them structurally using a photodiode array detector. With on-column preconcentration, we were able to measure low-ppb levels in human serum. Using blood samples from human volunteers, whose diet was supplemented by a soy-based product, the method has been validated for high-throughput screening of isoflavones in clinical studies.

Original languageEnglish (US)
Pages (from-to)5998-6005
Number of pages8
JournalAnalytical Chemistry
Issue number23
StatePublished - Dec 1 2002

ASJC Scopus subject areas

  • Analytical Chemistry

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