Development and characterization of a human marrow stromal cell line that enhances osteoclast-like cell formation

S. Takahashi, S. V. Reddy, M. Dallas, R. Devlin, J. Y. Chou, G. David Roodman

Research output: Contribution to journalArticle

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Abstract

We established a human bone marrow stromal cell line (Saka) by infecting marrow adherent cells from semisolid marrow cultures with a recombinant simian virus-40 (SV40) virus. The cells expressed SV40 large tumor antigen, had a fibroblast-like shape, and expressed fibronectin and vimentin. They did not contain detectable alkaline phosphatase activity; express myeloid, lymphoid, or factor VIII-associated antigens; or develop adipocyte-like characteristics with dexamethasone treatment. Polymerase chain reaction analysis of Saka cell RNA detected expression of messenger RNAs for interleukin-6 (IL-6), IL-1β, granulocyte-macrophage colony-stimulating factor, macrophage colony-stimulating factor, stem cell factor, and the 1,25- dihydroxyvitamin D3 receptor. Coculture of Saka cells with human marrow mononuclear cells enhanced formation of osteoclast-like multinucleated cells (MNC) in long term human bone marrow cultures. These MNC expressed calcitonin receptors and formed resorption lacunae on dentine. In contrast, coculture of marrow mononuclear cells with other SV40-transformed human marrow stromal cell lines did not increase MNC formation. Conditioned medium from Saka cells or coculture of bone marrow and Saka cells separated by a Millipore membrane did not enhance MNC formation. Addition of a neutralizing antibody to IL-6 or IL-1β blocked the effects of Saka cells on MNC formation. These results suggest that marrow stromal cells enhance osteoclast formation in part through direct cell to cell contact and production of IL-6 and/or IL-1β.

Original languageEnglish (US)
Pages (from-to)1441-1449
Number of pages9
JournalEndocrinology
Volume136
Issue number4
StatePublished - 1995
Externally publishedYes

Fingerprint

Osteoclasts
Stromal Cells
Bone Marrow
Cell Line
Simian virus 40
Coculture Techniques
Interleukin-1
Interleukin-6
Calcitonin Receptors
Calcitriol Receptors
Stem Cell Factor
Macrophage Colony-Stimulating Factor
Factor VIII
Neoplasm Antigens
Vimentin
Dentin
Granulocyte-Macrophage Colony-Stimulating Factor
Conditioned Culture Medium
Neutralizing Antibodies
Mesenchymal Stromal Cells

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Takahashi, S., Reddy, S. V., Dallas, M., Devlin, R., Chou, J. Y., & Roodman, G. D. (1995). Development and characterization of a human marrow stromal cell line that enhances osteoclast-like cell formation. Endocrinology, 136(4), 1441-1449.

Development and characterization of a human marrow stromal cell line that enhances osteoclast-like cell formation. / Takahashi, S.; Reddy, S. V.; Dallas, M.; Devlin, R.; Chou, J. Y.; Roodman, G. David.

In: Endocrinology, Vol. 136, No. 4, 1995, p. 1441-1449.

Research output: Contribution to journalArticle

Takahashi, S, Reddy, SV, Dallas, M, Devlin, R, Chou, JY & Roodman, GD 1995, 'Development and characterization of a human marrow stromal cell line that enhances osteoclast-like cell formation', Endocrinology, vol. 136, no. 4, pp. 1441-1449.
Takahashi, S. ; Reddy, S. V. ; Dallas, M. ; Devlin, R. ; Chou, J. Y. ; Roodman, G. David. / Development and characterization of a human marrow stromal cell line that enhances osteoclast-like cell formation. In: Endocrinology. 1995 ; Vol. 136, No. 4. pp. 1441-1449.
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