Development and comparison of a minimally-invasive model of autologous clot pulmonary embolism in Sprague-Dawley and Copenhagen rats

Michael S. Runyon, Michael A. Gellar, Nina Sanapareddy, Jeffrey Kline, John A. Watts

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Background: Experimental models of pulmonary embolism (PE) that produce pulmonary hypertension (PH) employ many different methods of inducing acute pulmonary occlusion. Many of these models induce PE with intravenous injection of exogenous impervious objects that may not completely reproduce the physiological properties of autologous thromboembolism. Current literature lacks a simple, well-described rat model of autlogous PE. Objective: Test if moderate-severity autologous PE in Sprague-Dawley (SD) and Copenhagen (Cop) rats can produce persistent PH.Methods: blood was withdrawn from the jugular vein, treated with thrombin-Ca++ and re-injected following pretreatment with tranexamic acid. Hemodynamic values, clot weights and biochemical measurements were performed at 1 and 5 days.Results: Infusion of clot significantly increased the right ventricular peak systolic pressure to 45-55 mm Hg, followed by normalization within 24 hours in SD rats, and within 5 days in COP rats. Clot lysis was 95% (24 hours) and 97% (5 days) in SD rats and was significantly lower in COP rats (70%, 24 hours; 87% 5 days). Plasma D-dimer was elevated in surgical sham animals and was further increased 8 hours after pulmonary embolism. Neither strain showed a significant increase in bronchoalveolar chemotactic activity, myeloperoxidase activity, leukocyte infiltration, or chemokine accumulation, indicating that there was no significant pulmonary inflammation.Conclusions: Both SD and COP rats exhibited near complete fibrinolysis of autologous clot PE within 5 days. Neither strain developed persistent PH. Experimental models of PE designed to induce sustained PH and a robust inflammatory response appear to require significant, persistent pulmonary vascular occlusion.

Original languageEnglish (US)
Article number3
JournalThrombosis Journal
Volume8
DOIs
StatePublished - Feb 11 2010
Externally publishedYes

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Pulmonary Embolism
Sprague Dawley Rats
Pulmonary Hypertension
Theoretical Models
Tranexamic Acid
Lung
Thromboembolism
Jugular Veins
Fibrinolysis
Chemokines
Thrombin
Intravenous Injections
Peroxidase
Blood Vessels
Pneumonia
Leukocytes
Hemodynamics
Blood Pressure
Weights and Measures

ASJC Scopus subject areas

  • Hematology

Cite this

Development and comparison of a minimally-invasive model of autologous clot pulmonary embolism in Sprague-Dawley and Copenhagen rats. / Runyon, Michael S.; Gellar, Michael A.; Sanapareddy, Nina; Kline, Jeffrey; Watts, John A.

In: Thrombosis Journal, Vol. 8, 3, 11.02.2010.

Research output: Contribution to journalArticle

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