Development of a femoral non-union model in the mouse

Matthew E. Oetgen, Greg A. Merrell, Nancy W. Troiano, Mark C. Horowitz, Melissa Kacena

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Objectives: Advancements in our knowledge of fracture healing have occurred in large part by the understanding of this process on a microscopic level. The ability to develop experimental non-union models in animals will assist in the investigation of this problem and are likely to lead to novel treatments. We report on a technique for developing experimental non-unions in mice. Methods: Femoral fractures were created in 48 CD1 mice, 24 mice underwent standard closed femoral fractures, and 24 mice underwent creation of a femoral non-union through an open osteotomy and fracture devascularisation method. All fractures were subsequently rodded. Histological examinations of the fractures were then conducted at eight time points post-operatively. Results: The control group showed normal fracture healing with histological evidence of bony fracture bridging by 28 days and mature bony remodelling at 63 days. The non-union group showed delayed fracture healing at all time points and no evidence of bony healing at 63 days. Conclusion: This is the first report of a reliable method to develop fracture non-union in mice. We believe this technique will be critical to further the investigation of fracture non-union in normal mice and provides the great advantage of using the plethora of transgenic and knockout mouse models to analyse non-union at the cell and molecular level.

Original languageEnglish (US)
Pages (from-to)1119-1126
Number of pages8
JournalInjury
Volume39
Issue number10
DOIs
StatePublished - Oct 2008
Externally publishedYes

Fingerprint

Thigh
Fracture Healing
Femoral Fractures
Closed Fractures
Open Fractures
Osteotomy
Knockout Mice
Transgenic Mice
Animal Models
Control Groups

Keywords

  • Experiment design
  • Mice
  • Non-union

ASJC Scopus subject areas

  • Emergency Medicine
  • Orthopedics and Sports Medicine

Cite this

Oetgen, M. E., Merrell, G. A., Troiano, N. W., Horowitz, M. C., & Kacena, M. (2008). Development of a femoral non-union model in the mouse. Injury, 39(10), 1119-1126. https://doi.org/10.1016/j.injury.2008.04.008

Development of a femoral non-union model in the mouse. / Oetgen, Matthew E.; Merrell, Greg A.; Troiano, Nancy W.; Horowitz, Mark C.; Kacena, Melissa.

In: Injury, Vol. 39, No. 10, 10.2008, p. 1119-1126.

Research output: Contribution to journalArticle

Oetgen, ME, Merrell, GA, Troiano, NW, Horowitz, MC & Kacena, M 2008, 'Development of a femoral non-union model in the mouse', Injury, vol. 39, no. 10, pp. 1119-1126. https://doi.org/10.1016/j.injury.2008.04.008
Oetgen ME, Merrell GA, Troiano NW, Horowitz MC, Kacena M. Development of a femoral non-union model in the mouse. Injury. 2008 Oct;39(10):1119-1126. https://doi.org/10.1016/j.injury.2008.04.008
Oetgen, Matthew E. ; Merrell, Greg A. ; Troiano, Nancy W. ; Horowitz, Mark C. ; Kacena, Melissa. / Development of a femoral non-union model in the mouse. In: Injury. 2008 ; Vol. 39, No. 10. pp. 1119-1126.
@article{24ebd1fb7f0b49d9ae8f13915cff8b49,
title = "Development of a femoral non-union model in the mouse",
abstract = "Objectives: Advancements in our knowledge of fracture healing have occurred in large part by the understanding of this process on a microscopic level. The ability to develop experimental non-union models in animals will assist in the investigation of this problem and are likely to lead to novel treatments. We report on a technique for developing experimental non-unions in mice. Methods: Femoral fractures were created in 48 CD1 mice, 24 mice underwent standard closed femoral fractures, and 24 mice underwent creation of a femoral non-union through an open osteotomy and fracture devascularisation method. All fractures were subsequently rodded. Histological examinations of the fractures were then conducted at eight time points post-operatively. Results: The control group showed normal fracture healing with histological evidence of bony fracture bridging by 28 days and mature bony remodelling at 63 days. The non-union group showed delayed fracture healing at all time points and no evidence of bony healing at 63 days. Conclusion: This is the first report of a reliable method to develop fracture non-union in mice. We believe this technique will be critical to further the investigation of fracture non-union in normal mice and provides the great advantage of using the plethora of transgenic and knockout mouse models to analyse non-union at the cell and molecular level.",
keywords = "Experiment design, Mice, Non-union",
author = "Oetgen, {Matthew E.} and Merrell, {Greg A.} and Troiano, {Nancy W.} and Horowitz, {Mark C.} and Melissa Kacena",
year = "2008",
month = "10",
doi = "10.1016/j.injury.2008.04.008",
language = "English (US)",
volume = "39",
pages = "1119--1126",
journal = "Injury",
issn = "0020-1383",
publisher = "Elsevier Limited",
number = "10",

}

TY - JOUR

T1 - Development of a femoral non-union model in the mouse

AU - Oetgen, Matthew E.

AU - Merrell, Greg A.

AU - Troiano, Nancy W.

AU - Horowitz, Mark C.

AU - Kacena, Melissa

PY - 2008/10

Y1 - 2008/10

N2 - Objectives: Advancements in our knowledge of fracture healing have occurred in large part by the understanding of this process on a microscopic level. The ability to develop experimental non-union models in animals will assist in the investigation of this problem and are likely to lead to novel treatments. We report on a technique for developing experimental non-unions in mice. Methods: Femoral fractures were created in 48 CD1 mice, 24 mice underwent standard closed femoral fractures, and 24 mice underwent creation of a femoral non-union through an open osteotomy and fracture devascularisation method. All fractures were subsequently rodded. Histological examinations of the fractures were then conducted at eight time points post-operatively. Results: The control group showed normal fracture healing with histological evidence of bony fracture bridging by 28 days and mature bony remodelling at 63 days. The non-union group showed delayed fracture healing at all time points and no evidence of bony healing at 63 days. Conclusion: This is the first report of a reliable method to develop fracture non-union in mice. We believe this technique will be critical to further the investigation of fracture non-union in normal mice and provides the great advantage of using the plethora of transgenic and knockout mouse models to analyse non-union at the cell and molecular level.

AB - Objectives: Advancements in our knowledge of fracture healing have occurred in large part by the understanding of this process on a microscopic level. The ability to develop experimental non-union models in animals will assist in the investigation of this problem and are likely to lead to novel treatments. We report on a technique for developing experimental non-unions in mice. Methods: Femoral fractures were created in 48 CD1 mice, 24 mice underwent standard closed femoral fractures, and 24 mice underwent creation of a femoral non-union through an open osteotomy and fracture devascularisation method. All fractures were subsequently rodded. Histological examinations of the fractures were then conducted at eight time points post-operatively. Results: The control group showed normal fracture healing with histological evidence of bony fracture bridging by 28 days and mature bony remodelling at 63 days. The non-union group showed delayed fracture healing at all time points and no evidence of bony healing at 63 days. Conclusion: This is the first report of a reliable method to develop fracture non-union in mice. We believe this technique will be critical to further the investigation of fracture non-union in normal mice and provides the great advantage of using the plethora of transgenic and knockout mouse models to analyse non-union at the cell and molecular level.

KW - Experiment design

KW - Mice

KW - Non-union

UR - http://www.scopus.com/inward/record.url?scp=50249099181&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=50249099181&partnerID=8YFLogxK

U2 - 10.1016/j.injury.2008.04.008

DO - 10.1016/j.injury.2008.04.008

M3 - Article

VL - 39

SP - 1119

EP - 1126

JO - Injury

JF - Injury

SN - 0020-1383

IS - 10

ER -