Development of a native plasmid as a cloning vector in clavibacter xyli subsp. Cynodontis

Jeffrey Taylor, Robert S. Stearman, Brenda B. Uratani

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

A 50-kilobase (kb) cryptic plasmid was found in three geographic isolates of Clavibacter xyli subsp. cynodontis (Cxc). The DNA region essential for replication of the plasmid was cloned in an Escherichia coli vector. The resulting vector, which functions as a shuttle vector between E. coli and Cxc, was characterized further with respect to its stability and copy number.

Original languageEnglish (US)
Pages (from-to)241-244
Number of pages4
JournalPlasmid
Volume29
Issue number3
DOIs
StatePublished - May 1993
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology

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