Development of a porcine delayed wound-healing model and its use in testing a novel cell-based therapy

Ivan Hadad, Brian H. Johnstone, Jeffrey G. Brabham, Matthew W. Blanton, Pamela I. Rogers, Cory Fellers, James L. Solomon, Stephanie Merfeld-Clauss, Colleen M. Desrosiers, Joseph R. Dynlacht, John J. Coleman, Keith L. March

Research output: Contribution to journalArticle

34 Scopus citations


Purpose: A delayed full-thickness wound-healing model was developed and used for examining the capacity of adipose-derived stem cells (ASCs), either alone or in platelet-rich fibrin gels, to promote healing. Methods and Materials: Four pigs received electron beam radiation to the dorsal skin surface. Five weeks after radiation, subcutaneous fat was harvested from nonirradiated areas and processed to yield ASCs. Two weeks later, 28 to 30 full-thickness 1.5-cm2 wounds were made in irradiated and nonirradiated skin. Wounds were treated with either saline solution, ASCs in saline solution, platelet-rich plasma (PRP) fibrin gel, ASCs in PRP, or non-autologous green fluorescence protein-labeled ASCs. Results: The single radiation dose produced a significant loss of dermal microvasculature density (75%) by 7 weeks. There was a significant difference in the rate of healing between irradiated and nonirradiated skin treated with saline solution. The ASCs in PRP-treated wounds exhibited a significant 11.2% improvement in wound healing compared with saline solution. Enhancement was dependent on the combination of ASCs and PRP, because neither ASCs nor PRP alone had an effect. Conclusions: We have created a model that simulates the clinically relevant late radiation effects of delayed wound healing. Using this model, we showed that a combination of ASCs and PRP improves the healing rates of perfusion-depleted tissues, possibly through enhancing local levels of growth factors.

Original languageEnglish (US)
Pages (from-to)888-896
Number of pages9
JournalInternational Journal of Radiation Oncology Biology Physics
Issue number3
StatePublished - Aug 13 2010


  • Adult stem cells
  • Disease models
  • Radiation injuries
  • Wound healing

ASJC Scopus subject areas

  • Oncology
  • Radiology Nuclear Medicine and imaging
  • Radiation
  • Cancer Research
  • Medicine(all)

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    Hadad, I., Johnstone, B. H., Brabham, J. G., Blanton, M. W., Rogers, P. I., Fellers, C., Solomon, J. L., Merfeld-Clauss, S., Desrosiers, C. M., Dynlacht, J. R., Coleman, J. J., & March, K. L. (2010). Development of a porcine delayed wound-healing model and its use in testing a novel cell-based therapy. International Journal of Radiation Oncology Biology Physics, 78(3), 888-896.