Development of a transgenic mouse that overexpresses a novel product of the growth hormone-releasing hormone gene

S. Fang, R. Steinmetz, D. W. King, P. Zeng, C. Vogelweid, S. Cooper, G. Hangcoc, H. E. Broxmeyer, O. H. Pescovitz

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

The GH-releasing hormone (GHRH) precursor molecule contains a 30-amino acid C-terminal region that has been designated GHRH-related peptide (GHRH-RP). To begin to understand the physiological role of GHRH-RP, transgenic (Tg) mice that constituitively express this peptide were developed. To generate these mice, a transgene (SS-RP) was constructed by overlap primer extension PCR. This transgene, under the control of the mouse phosphoglycerate kinase gene, selectively expresses GHRH-RP, but not GHRH. Western blot analysis confirmed that the transgene produces GHRH-RP. Animals were evaluated for the effect of excess GHRH-RP on growth, fertility, behavior, stem cell factor (SCF) expression, and hematopoiesis. Northern blot and RT-PCR were used to demonstrate ubiquitous expression of the transgene in tissues from GHRH-RP Tg animals. These tissues also had marked overexpression of SCF messenger RNA compared with controls. Tg animals had significantly increased cell cycling for granulocyte-macrophage, erythroid, and multilineage progenitor cells. Transgenic animals did not differ from control mice in their growth, fertility, or behavior. These findings demonstrate, for the first time, that in vivo the C-terminal peptide of the pro-GHRH molecule is a biologically active peptide that is capable of stimulating the expression of SCF and hematopoiesis in vivo and suggests that GHRH-RP may play a role in normal blood cell development.

Original languageEnglish (US)
Pages (from-to)1377-1383
Number of pages7
JournalEndocrinology
Volume141
Issue number4
DOIs
StatePublished - Jan 1 2000

ASJC Scopus subject areas

  • Endocrinology

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    Fang, S., Steinmetz, R., King, D. W., Zeng, P., Vogelweid, C., Cooper, S., Hangcoc, G., Broxmeyer, H. E., & Pescovitz, O. H. (2000). Development of a transgenic mouse that overexpresses a novel product of the growth hormone-releasing hormone gene. Endocrinology, 141(4), 1377-1383. https://doi.org/10.1210/endo.141.4.7422