Development of acute tolerance during steady-state arterial alcohol concentrations: A study of auditory event-related potentials in rats

Sandra L. Morzorati, Robert B. Stewart

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Background: The blood alcohol clamp is a method whereby alcohol is infused intravenously to maintain a predetermined arterial alcohol concentration (AAC) for an indefinite period of time. The objective of this study was to use the clamp to examine the effects of alcohol on event-related potentials (ERPs) in rats and to assess the development of tolerance during a single alcohol exposure. Methods: Adult male Wistar rats that had a chronic implant of EEG electrodes overlying the frontal cortex and were equipped with cannulae in the jugular vein, were clamped at 75 or 150 mg/dl via an intravenous infusion of 20% (v/v) alcohol. Auditory ERPs were recorded before the alcohol infusion (baseline) and at 5, 15, 120, 135, or 195 min after steady-state AAC was achieved. In a separate group of rats, test-retest reliability was examined by acquiring ERPs two to three times in the same rat at 60-min intervals. Dependent variables were calculated as changes from baseline for each time point for P1-N1 amplitude and P1 and N1 latencies. Results: In the test-retest study, there were no differences in any of the dependent variables over time, indicating that the measures were stable and repeatable. Estimated AACs of 75 and 150 mg/dl significantly (p = 0.0001) decreased P1-N1 amplitude in a dose-related manner. During both clamps, the alcohol effect peaked at 120 min (p < 0.03) and decreased thereafter. Alcohol had no effect on P1 or N1 latencies. Conclusions: Pharmacologically relevant AACs significantly decreased the amplitude but not the latencies of the long-latency components of the rat auditory ERP. Acute tolerance developed because the amplitude of the ERP component recovered as AACs were held relatively constant.

Original languageEnglish (US)
Pages (from-to)347-352
Number of pages6
JournalAlcoholism: Clinical and Experimental Research
Volume29
Issue number3
DOIs
StatePublished - Mar 1 2005

Keywords

  • Acute Tolerance to Alcohol
  • Auditory Event-Related Potentials
  • Blood Alcohol Clamp
  • Rats

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Toxicology

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