Development of hammerhead ribozymes to modulate endogenous gene expression for functional studies

Jason Jon Fritz, Alfred Lewin, William Hauswirth, Anupam Agarwal, Maria Grant, Lynn Shaw

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Hammerhead ribozymes are catalytic RNAs that are being used to inhibit endogenous gene expression to study key components of basic biochemical pathways such as angiogenesis. In addition, these ribozymes have the potential to be used as components of gene therapy protocols for the treatment of disease states. We detail here a set of protocols for the design and testing of hammerhead ribozymes that will efficiently inhibit gene expression both in cell culture and in vivo.

Original languageEnglish (US)
Pages (from-to)276-285
Number of pages10
JournalMethods
Volume28
Issue number2
DOIs
StatePublished - 2002
Externally publishedYes

Fingerprint

Catalytic RNA
Gene expression
Gene Expression
Gene therapy
Clinical Protocols
Cell culture
Genetic Therapy
Cell Culture Techniques
Testing
hammerhead ribozyme

Keywords

  • Endothelial cell
  • Gene therapy
  • Hammerhead ribozyme
  • Kinetic analysis
  • Transfection

ASJC Scopus subject areas

  • Molecular Biology

Cite this

Fritz, J. J., Lewin, A., Hauswirth, W., Agarwal, A., Grant, M., & Shaw, L. (2002). Development of hammerhead ribozymes to modulate endogenous gene expression for functional studies. Methods, 28(2), 276-285. https://doi.org/10.1016/S1046-2023(02)00233-5

Development of hammerhead ribozymes to modulate endogenous gene expression for functional studies. / Fritz, Jason Jon; Lewin, Alfred; Hauswirth, William; Agarwal, Anupam; Grant, Maria; Shaw, Lynn.

In: Methods, Vol. 28, No. 2, 2002, p. 276-285.

Research output: Contribution to journalArticle

Fritz, JJ, Lewin, A, Hauswirth, W, Agarwal, A, Grant, M & Shaw, L 2002, 'Development of hammerhead ribozymes to modulate endogenous gene expression for functional studies', Methods, vol. 28, no. 2, pp. 276-285. https://doi.org/10.1016/S1046-2023(02)00233-5
Fritz, Jason Jon ; Lewin, Alfred ; Hauswirth, William ; Agarwal, Anupam ; Grant, Maria ; Shaw, Lynn. / Development of hammerhead ribozymes to modulate endogenous gene expression for functional studies. In: Methods. 2002 ; Vol. 28, No. 2. pp. 276-285.
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