Development of hammerhead ribozymes to modulate endogenous gene expression for functional studies

Jason Jon Fritz, Alfred Lewin, William Hauswirth, Anupam Agarwal, Maria Grant, Lynn Shaw

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

Hammerhead ribozymes are catalytic RNAs that are being used to inhibit endogenous gene expression to study key components of basic biochemical pathways such as angiogenesis. In addition, these ribozymes have the potential to be used as components of gene therapy protocols for the treatment of disease states. We detail here a set of protocols for the design and testing of hammerhead ribozymes that will efficiently inhibit gene expression both in cell culture and in vivo.

Original languageEnglish (US)
Pages (from-to)276-285
Number of pages10
JournalMethods
Volume28
Issue number2
DOIs
StatePublished - Nov 26 2002

Keywords

  • Endothelial cell
  • Gene therapy
  • Hammerhead ribozyme
  • Kinetic analysis
  • Transfection

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)

Fingerprint Dive into the research topics of 'Development of hammerhead ribozymes to modulate endogenous gene expression for functional studies'. Together they form a unique fingerprint.

Cite this