Development of inward rectification and control of membrane excitability in mesencephalic V neurons

Susumu Tanaka, Nanping Wu, Chie Fang Hsaio, Jack Turman, Scott H. Chandler

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

The present study was performed to assess the postnatal development and functional roles of inward rectifying currents in rat mesencephalic trigeminal (Mes V) neurons, which are involved in the genesis and control of oral-motor activities. Whole cell voltage-clamp recordings obtained from Mes V neurons in brain stem slices identified fast (IKIR) and slow (Ih) inward rectifying currents, which were specifically blocked by BaCl2 (300-500 μM) or 4-(N-ethyl-N-phenylamino)-1,2-dimethyl-6-(methylamino) pyrimidinium chloride (ZD 7288, 10 μM), respectively. The whole cell current density for these channels increased between postnatal days 2 to 12 (P2-P12), and the time courses for Ih activation and deactivation were each well described by two time constants. Application of ZD 7288 produced membrane hyperpolarization in the majority of cells and prolonged afterhyperpolarization repolarization. Additionally, in the presence of ZD 7288, spike frequency was decreased and adaptation was more pronounced. Interestingly, these neurons exhibited a voltage-dependent membrane resonance (<10 Hz) that was prominent around resting potential and more negative to rest and was blocked by ZD 7288. These results suggest that Ih contributes to stabilizing resting membrane potential and controlling cell excitability. The presence of Ih imparts the neuron with the unique property of low-frequency membrane resonance; the ability to discriminate between synaptic inputs based on frequency content.

Original languageEnglish (US)
Pages (from-to)1288-1298
Number of pages11
JournalJournal of Neurophysiology
Volume89
Issue number3
DOIs
StatePublished - Mar 1 2003
Externally publishedYes

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Neurons
Membranes
Membrane Potentials
Aptitude
Brain Stem
Motor Activity
Cell Count
ICI D2788

ASJC Scopus subject areas

  • Physiology
  • Neuroscience(all)

Cite this

Development of inward rectification and control of membrane excitability in mesencephalic V neurons. / Tanaka, Susumu; Wu, Nanping; Hsaio, Chie Fang; Turman, Jack; Chandler, Scott H.

In: Journal of Neurophysiology, Vol. 89, No. 3, 01.03.2003, p. 1288-1298.

Research output: Contribution to journalArticle

Tanaka, Susumu ; Wu, Nanping ; Hsaio, Chie Fang ; Turman, Jack ; Chandler, Scott H. / Development of inward rectification and control of membrane excitability in mesencephalic V neurons. In: Journal of Neurophysiology. 2003 ; Vol. 89, No. 3. pp. 1288-1298.
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