Development of Multiorgan Pathology in the wpk Rat Model of Polycystic Kidney Disease

Vincent H. Gattone, Benjamin A. Tourkow, Chad M. Trambaugh, Alexander C. Yu, Shelly Whelan, Carrie Phillips, Peter C. Harris, Richard G. Peterson

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Rodent models of polycystic kidney disease (PKD) have provided valuable insight into the cellular changes associated with cystogenesis in humans. The present study characterizes the morphology of renal and extrarenal pathology of autosomal recessive PKD induced by the wpk gene in Wistar rats. In wpk -/- rats, proximal tubule and collecting duct cysts develop in utero and eventually consume the kidney. Increased apoptosis, mitosis, and extracellular tenascin deposition parallel cyst development. Extrarenal pathology occurs in the immune system (thymic and splenic hypoplasia) and central nervous system (CNS; hypoplasia to agenesis of the corpus callosum with severe hydrocephalus). Severity of hydrocephalus varied inversely with size of the corpus callosum. In wpk-/- rats, the corpus callosum exhibits relatively few axons that cross the midline. This CNS pathology is similar to that described in three human renal cystic syndromes: orofaciodigital, genitopatellar, and cerebrorenal-digital syndromes. Collecting duct and ventricular ependymal cilia appear morphologically normal. To determine if rodent background strain and the presence of modifier genes affect severity of the disease, we crossed the Wistar-wpk rat with Brown Norway (BN) and Long Evan (LE) rats and found the degree of renal and cerebral pathology was diminished as evidenced by lower kidney weight as a percent of body weight and serum urea nitrogen concentration in cystic rats on LE or BN strains as well as less prominent cranial enlargement. Crosses with BN rats allowed us to localize the wpk gene on chromosome 5 very close to the D5Rat73 marker. The wpk gene lies within a chromosomal region known to harbor a PKD modifier locus. In summary, the types of renal and cerebral pathology seen in the Wistar wpk rat are a unique combination seen only in this rodent model.

Original languageEnglish
Pages (from-to)384-395
Number of pages12
JournalAnatomical Record - Part A Discoveries in Molecular, Cellular, and Evolutionary Biology
Volume277
Issue number2
StatePublished - Apr 2004

Fingerprint

Polycystic Kidney Diseases
animal models
Pathology
Kidney
kidneys
rats
Wistar Rats
Rodentia
Long Evans Rats
Corpus Callosum
hydrocephalus
Norway
Hydrocephalus
rodents
modifiers (genes)
Cysts
Orofaciodigital Syndromes
Autosomal Recessive Polycystic Kidney
Modifier Genes
Agenesis of Corpus Callosum

Keywords

  • Cilia
  • Corpus callosum
  • Hydrocephalus
  • Polycystic kidney disease
  • Rat chromosome 5

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Anatomy

Cite this

Development of Multiorgan Pathology in the wpk Rat Model of Polycystic Kidney Disease. / Gattone, Vincent H.; Tourkow, Benjamin A.; Trambaugh, Chad M.; Yu, Alexander C.; Whelan, Shelly; Phillips, Carrie; Harris, Peter C.; Peterson, Richard G.

In: Anatomical Record - Part A Discoveries in Molecular, Cellular, and Evolutionary Biology, Vol. 277, No. 2, 04.2004, p. 384-395.

Research output: Contribution to journalArticle

Gattone, VH, Tourkow, BA, Trambaugh, CM, Yu, AC, Whelan, S, Phillips, C, Harris, PC & Peterson, RG 2004, 'Development of Multiorgan Pathology in the wpk Rat Model of Polycystic Kidney Disease', Anatomical Record - Part A Discoveries in Molecular, Cellular, and Evolutionary Biology, vol. 277, no. 2, pp. 384-395.
Gattone, Vincent H. ; Tourkow, Benjamin A. ; Trambaugh, Chad M. ; Yu, Alexander C. ; Whelan, Shelly ; Phillips, Carrie ; Harris, Peter C. ; Peterson, Richard G. / Development of Multiorgan Pathology in the wpk Rat Model of Polycystic Kidney Disease. In: Anatomical Record - Part A Discoveries in Molecular, Cellular, and Evolutionary Biology. 2004 ; Vol. 277, No. 2. pp. 384-395.
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