Development, validation and implementation of radio-HPLC methods for the P2X7-receptor-targeted [11C]GSK1482160 radiopharmaceutical

Carmen L. Wissmann, Min Wang, Mingzhang Gao, Qi-Huang Zheng, Mark Green

Research output: Contribution to journalArticle

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A radio-analytical RP-HPLC method was developed and validated to support production of the P2X7-receptor-targeted [11C]GSK1482160 radiopharmaceutical. Method validation included characterization of retention times, peak shapes, linearity, accuracy, precision, selectivity, limits of detection and quantitation (UV signal), radiochemical stability, as well as analytical method range and robustness. The validated radio-HPLC method is suitable for the definition of [11C]GSK1482160 radiochemical identity, radiochemical purity, as well as molar activity, and is being employed in support of human studies with [11C]GSK1482160.

Original languageEnglish (US)
Pages (from-to)8-11
Number of pages4
JournalApplied Radiation and Isotopes
StatePublished - Dec 1 2018



  • Inflammation
  • P2X7 receptor
  • Radio-analytical HPLC method
  • [C]GSK1482160

ASJC Scopus subject areas

  • Radiation

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