Developmental association of the synaptic activity-regulated protein arc with the mouse acrosomal organelle and the sperm tail

Bernhard Maier, Silvia Medrano, Susan B. Sleight, Pablo E. Visconti, Heidi Scrable

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

In neurons, arc (activity regulated, cytoskeleton associated) is an immediate early gene (IEG) that is rapidly and transiently induced by excitatory stimulation. It is believed to mediate rapid strengthening of signaling structures at activated synaptic sites. Unlike most IEGs, arc does not encode nuclear transcription factor, but an effector molecule that associates with the actin cytoskeleton. Cytoskeletal rearrangements of microtubule- and actin-based networks that occur at activated synapses also take place in similar fashion during the formation of the acrosome, the site of regulated exocytosis at fertilization. In this paper, arc is reported to be highly expressed both at the RNA and protein levels in postmeiotic germ cells in the testis of adult mice. Immunofluorescence studies reveal that arc is first present in the perinuclear region of round, elongating, and elongate spermatids, where it colocalizes with the developing acrosome. In isolated mature sperm, arc is present in the acrosomal region of the sperm head, the centriole region of the neck, and the principal piece of the tail. Arc is lost to varying degrees during sperm capacitation and in acrosome-reacted sperm. Phalloidin staining of mature sperm cells reveals an overlapping pattern of filamentous-actin and arc expression. These results support a role for arc and the actin cytoskeleton in the acrosome formation, the sperm acrosome reaction, and in sperm cell motility.

Original languageEnglish (US)
Pages (from-to)67-76
Number of pages10
JournalBiology of Reproduction
Volume68
Issue number1
DOIs
StatePublished - Jan 1 2003
Externally publishedYes

Fingerprint

Sperm Tail
Cytoskeleton
Organelles
Acrosome
Spermatozoa
Actin Cytoskeleton
Actins
Sperm Capacitation
Centrioles
Sperm Head
Acrosome Reaction
Phalloidine
Immediate-Early Genes
Spermatids
Sperm Motility
Exocytosis
activity regulated cytoskeletal-associated protein
Fertilization
Germ Cells
Microtubules

Keywords

  • Acrosome reaction
  • Gametogenesis
  • Sperm capacitation
  • Sperm motility and transport
  • Spermatogenesis

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Embryology

Cite this

Developmental association of the synaptic activity-regulated protein arc with the mouse acrosomal organelle and the sperm tail. / Maier, Bernhard; Medrano, Silvia; Sleight, Susan B.; Visconti, Pablo E.; Scrable, Heidi.

In: Biology of Reproduction, Vol. 68, No. 1, 01.01.2003, p. 67-76.

Research output: Contribution to journalArticle

Maier, Bernhard ; Medrano, Silvia ; Sleight, Susan B. ; Visconti, Pablo E. ; Scrable, Heidi. / Developmental association of the synaptic activity-regulated protein arc with the mouse acrosomal organelle and the sperm tail. In: Biology of Reproduction. 2003 ; Vol. 68, No. 1. pp. 67-76.
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