Dexamethasone stimulates leptin release from human adipocytes: Unexpected inhibition by insulin

Robert V. Considine, Mark R. Nyce, Jerzy W. Kolaczynski, Pei Li Zhang, Joanna P. Ohannesian, John H. Moore, James W. Fox, José F. Caro

Research output: Contribution to journalArticle

86 Scopus citations

Abstract

In the present study we have examined the effect of dexamethasone on ob gene mRNA expression and leptin release from isolated human subcutaneous adipocytes. Dexamethasone stimulated leptin release from cultured adipocytes in a time- and dose-dependent manner. A two-fold increase in leptin release was detectable by 36 h of treatment with 10-7 M dexamethasone. Leptin release was preceded by a significant 83 ±30% increase in ob mRNA after 24 h exposure to the compound. Co-incubation of cells with dexamethasone (10- 7 M) and insulin (10-7 or 10-9 M) completely blocked the dexamethasone- stimulated increase in ob mRNA and leptin release. These data demonstrate that insulin and glucocorticoids regulate leptin synthesis and release from human adipocytes in vitro.

Original languageEnglish (US)
Pages (from-to)254-258
Number of pages5
JournalJournal of Cellular Biochemistry
Volume65
Issue number2
DOIs
StatePublished - May 1 1997

Keywords

  • human adipocytes
  • leptin
  • ob gene

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology

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