DhhP, a cyclic di-AMP phosphodiesterase of Borrelia burgdorferi, is essential for cell growth and virulence

Meiping Ye, Jun Jie Zhang, Xin Fang, Gavin B. Lawlis, Bryan Troxell, Yan Zhou, Mark Gomelsky, Yongliang Lou, X. Yang

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Cyclic di-AMP (c-di-AMP) is a recently discovered second messenger in bacteria. Most of work on c-di-AMP signaling has been done in Gram-positive bacteria, firmicutes, and actinobacteria, where c-di-AMP signaling pathways affect potassium transport, cell wall structure, and antibiotic resistance. Little is known about c-di-AMP signaling in other bacteria. Borrelia burgdorferi, the causative agent of Lyme disease, is a spirochete that has a Gram-negative dual membrane. In this study, we demonstrated that B. burgdorferi BB0619, a DHH-DHHA1 domain protein (herein designated DhhP), functions as c-di-AMP phosphodiesterase. Recombinant DhhP hydrolyzed c-di-AMP to pApA in a Mn2+ -or Mg2+-dependent manner. In contrast to c-di-AMP phosphodiesterases reported thus far, DhhP appears to be essential for B. burgdorferi growth both in vitro and in the mammalian host. Inactivation of the chromosomal dhhP gene could be achieved only in the presence of a plasmid-encoded inducible dhhP gene. The conditional dhhP mutant had a dramatic increase in intracellular c-di-AMP level in comparison to the isogenic wild-type strain. Unlike what has been observed in Gram-positive bacteria, elevated cellular c-di-AMP in B. burgdorferi did not result in an increased resistance to β-lactamase antibiotics, suggesting that c-di-AMP's functions in spirochetes differ from those in Grampositive bacteria. In addition, the dhhP mutant was defective in induction of the σS factor, RpoS, and the RpoS-dependent outer membrane virulence factor OspC, which uncovers an important role of c-di-AMP in B. burgdorferi virulence.

Original languageEnglish
Pages (from-to)1840-1849
Number of pages10
JournalInfection and Immunity
Volume82
Issue number5
DOIs
StatePublished - 2014

Fingerprint

Borrelia burgdorferi
Phosphoric Diester Hydrolases
Cyclic AMP
Virulence
Growth
Spirochaetales
Gram-Positive Bacteria
Bacteria
Membranes
Lyme Disease
Actinobacteria
Second Messenger Systems
Virulence Factors
Microbial Drug Resistance
Cell Wall
Genes
Potassium
Plasmids
Anti-Bacterial Agents

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Parasitology
  • Infectious Diseases

Cite this

DhhP, a cyclic di-AMP phosphodiesterase of Borrelia burgdorferi, is essential for cell growth and virulence. / Ye, Meiping; Zhang, Jun Jie; Fang, Xin; Lawlis, Gavin B.; Troxell, Bryan; Zhou, Yan; Gomelsky, Mark; Lou, Yongliang; Yang, X.

In: Infection and Immunity, Vol. 82, No. 5, 2014, p. 1840-1849.

Research output: Contribution to journalArticle

Ye, M, Zhang, JJ, Fang, X, Lawlis, GB, Troxell, B, Zhou, Y, Gomelsky, M, Lou, Y & Yang, X 2014, 'DhhP, a cyclic di-AMP phosphodiesterase of Borrelia burgdorferi, is essential for cell growth and virulence', Infection and Immunity, vol. 82, no. 5, pp. 1840-1849. https://doi.org/10.1128/IAI.00030-14
Ye, Meiping ; Zhang, Jun Jie ; Fang, Xin ; Lawlis, Gavin B. ; Troxell, Bryan ; Zhou, Yan ; Gomelsky, Mark ; Lou, Yongliang ; Yang, X. / DhhP, a cyclic di-AMP phosphodiesterase of Borrelia burgdorferi, is essential for cell growth and virulence. In: Infection and Immunity. 2014 ; Vol. 82, No. 5. pp. 1840-1849.
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AU - Lawlis, Gavin B.

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AU - Zhou, Yan

AU - Gomelsky, Mark

AU - Lou, Yongliang

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