Diabetes and hyperlipidemia correlate with gallbladder contractility in leptin-related murine obesity

Khoi Q. Tran, Matthew I. Goldblatt, Deborah A. Swartz-Basile, Carol Svatek, Attila Nakeeb, Henry A. Pitt

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Obesity is associated with many comorbid conditions including diabetes, hyperlipidemia, and gallstones. However, the interaction among these modalities remains unclear. We recently demonstrated that both leptin-deficient and leptin-resistant obese mice have impaired biliary motility. These obese mice also are diabetic and hyperlipidemic. Therefore, we tested the hypothesis that serum glucose, insulin, cholesterol, and triglyceride levels would correlate with gallbladder contractility. Thirty-four lean control, 10 lean heterozygous leptin-deficient, 18 obese homozygous leptin-deficient, and 12 obese homozygous leptin-resistant mice were fed a nonlithogenic chow diet while nine lean control and nine obese homozygous leptin-deficient mice were fed a high-cholesterol diet for 4 weeks. In vitro gallbladder responses to cholecystokinin (CCK; 10-8 mol/L), acetylcholine (ACh; 10 -5 mol/L), and neuropeptide Y (NPY; 10-6 mol/L) were measured. Serum glucose, insulin, cholesterol, and triglyceride levels were measured from pooled serum from an additional 704 animals. Gallbladder responses were greatest for CCK, intermediate for ACh, and least for NPY. Serum glucose, insulin, cholesterol, and triglyceride levels and body weight all correlated similarly, negatively, and significantly (P

Original languageEnglish (US)
Pages (from-to)857-863
Number of pages7
JournalJournal of Gastrointestinal Surgery
Volume7
Issue number7
DOIs
StatePublished - Nov 2003
Externally publishedYes

Fingerprint

Leptin
Hyperlipidemias
Gallbladder
Obesity
Cholesterol
Obese Mice
Triglycerides
Insulin
Serum
Glucose
Diet
Sincalide
Neuropeptide Y
Cholecystokinin
Gallstones
Acetylcholine
Body Weight

Keywords

  • Cholesterol
  • Diabetes
  • Gallbladder
  • Leptin
  • Obesity

ASJC Scopus subject areas

  • Surgery

Cite this

Diabetes and hyperlipidemia correlate with gallbladder contractility in leptin-related murine obesity. / Tran, Khoi Q.; Goldblatt, Matthew I.; Swartz-Basile, Deborah A.; Svatek, Carol; Nakeeb, Attila; Pitt, Henry A.

In: Journal of Gastrointestinal Surgery, Vol. 7, No. 7, 11.2003, p. 857-863.

Research output: Contribution to journalArticle

Tran, Khoi Q. ; Goldblatt, Matthew I. ; Swartz-Basile, Deborah A. ; Svatek, Carol ; Nakeeb, Attila ; Pitt, Henry A. / Diabetes and hyperlipidemia correlate with gallbladder contractility in leptin-related murine obesity. In: Journal of Gastrointestinal Surgery. 2003 ; Vol. 7, No. 7. pp. 857-863.
@article{ffe693acdd0d47c6bfd804394e13c944,
title = "Diabetes and hyperlipidemia correlate with gallbladder contractility in leptin-related murine obesity",
abstract = "Obesity is associated with many comorbid conditions including diabetes, hyperlipidemia, and gallstones. However, the interaction among these modalities remains unclear. We recently demonstrated that both leptin-deficient and leptin-resistant obese mice have impaired biliary motility. These obese mice also are diabetic and hyperlipidemic. Therefore, we tested the hypothesis that serum glucose, insulin, cholesterol, and triglyceride levels would correlate with gallbladder contractility. Thirty-four lean control, 10 lean heterozygous leptin-deficient, 18 obese homozygous leptin-deficient, and 12 obese homozygous leptin-resistant mice were fed a nonlithogenic chow diet while nine lean control and nine obese homozygous leptin-deficient mice were fed a high-cholesterol diet for 4 weeks. In vitro gallbladder responses to cholecystokinin (CCK; 10-8 mol/L), acetylcholine (ACh; 10 -5 mol/L), and neuropeptide Y (NPY; 10-6 mol/L) were measured. Serum glucose, insulin, cholesterol, and triglyceride levels were measured from pooled serum from an additional 704 animals. Gallbladder responses were greatest for CCK, intermediate for ACh, and least for NPY. Serum glucose, insulin, cholesterol, and triglyceride levels and body weight all correlated similarly, negatively, and significantly (P",
keywords = "Cholesterol, Diabetes, Gallbladder, Leptin, Obesity",
author = "Tran, {Khoi Q.} and Goldblatt, {Matthew I.} and Swartz-Basile, {Deborah A.} and Carol Svatek and Attila Nakeeb and Pitt, {Henry A.}",
year = "2003",
month = "11",
doi = "10.1016/S1091-255X(03)00146-X",
language = "English (US)",
volume = "7",
pages = "857--863",
journal = "Journal of Gastrointestinal Surgery",
issn = "1091-255X",
publisher = "Springer New York",
number = "7",

}

TY - JOUR

T1 - Diabetes and hyperlipidemia correlate with gallbladder contractility in leptin-related murine obesity

AU - Tran, Khoi Q.

AU - Goldblatt, Matthew I.

AU - Swartz-Basile, Deborah A.

AU - Svatek, Carol

AU - Nakeeb, Attila

AU - Pitt, Henry A.

PY - 2003/11

Y1 - 2003/11

N2 - Obesity is associated with many comorbid conditions including diabetes, hyperlipidemia, and gallstones. However, the interaction among these modalities remains unclear. We recently demonstrated that both leptin-deficient and leptin-resistant obese mice have impaired biliary motility. These obese mice also are diabetic and hyperlipidemic. Therefore, we tested the hypothesis that serum glucose, insulin, cholesterol, and triglyceride levels would correlate with gallbladder contractility. Thirty-four lean control, 10 lean heterozygous leptin-deficient, 18 obese homozygous leptin-deficient, and 12 obese homozygous leptin-resistant mice were fed a nonlithogenic chow diet while nine lean control and nine obese homozygous leptin-deficient mice were fed a high-cholesterol diet for 4 weeks. In vitro gallbladder responses to cholecystokinin (CCK; 10-8 mol/L), acetylcholine (ACh; 10 -5 mol/L), and neuropeptide Y (NPY; 10-6 mol/L) were measured. Serum glucose, insulin, cholesterol, and triglyceride levels were measured from pooled serum from an additional 704 animals. Gallbladder responses were greatest for CCK, intermediate for ACh, and least for NPY. Serum glucose, insulin, cholesterol, and triglyceride levels and body weight all correlated similarly, negatively, and significantly (P

AB - Obesity is associated with many comorbid conditions including diabetes, hyperlipidemia, and gallstones. However, the interaction among these modalities remains unclear. We recently demonstrated that both leptin-deficient and leptin-resistant obese mice have impaired biliary motility. These obese mice also are diabetic and hyperlipidemic. Therefore, we tested the hypothesis that serum glucose, insulin, cholesterol, and triglyceride levels would correlate with gallbladder contractility. Thirty-four lean control, 10 lean heterozygous leptin-deficient, 18 obese homozygous leptin-deficient, and 12 obese homozygous leptin-resistant mice were fed a nonlithogenic chow diet while nine lean control and nine obese homozygous leptin-deficient mice were fed a high-cholesterol diet for 4 weeks. In vitro gallbladder responses to cholecystokinin (CCK; 10-8 mol/L), acetylcholine (ACh; 10 -5 mol/L), and neuropeptide Y (NPY; 10-6 mol/L) were measured. Serum glucose, insulin, cholesterol, and triglyceride levels were measured from pooled serum from an additional 704 animals. Gallbladder responses were greatest for CCK, intermediate for ACh, and least for NPY. Serum glucose, insulin, cholesterol, and triglyceride levels and body weight all correlated similarly, negatively, and significantly (P

KW - Cholesterol

KW - Diabetes

KW - Gallbladder

KW - Leptin

KW - Obesity

UR - http://www.scopus.com/inward/record.url?scp=0242365576&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0242365576&partnerID=8YFLogxK

U2 - 10.1016/S1091-255X(03)00146-X

DO - 10.1016/S1091-255X(03)00146-X

M3 - Article

VL - 7

SP - 857

EP - 863

JO - Journal of Gastrointestinal Surgery

JF - Journal of Gastrointestinal Surgery

SN - 1091-255X

IS - 7

ER -