Diabetes related increase in plasminogen activator inhibitor-1 expression in monkey retinal capillaries

M. B. Grant, P. E. Spoerri

Research output: Contribution to journalArticle

Abstract

Plasminogen activator inhibitor-1 (PAI-1), a product of endothelial cells, is a regulator of fibrinolysis and a modulator of extracellular matrix proteolysis. Presently, we investigated PAI-1 expression in retinal capillaries of monkeys with noninsulin dependent diabetes mellitus (NIDDM) compared to nondiabetic controls. Colloidal gold immunocytochemistry was used to quantify PAI-1 antigen in retinas from diabetic (n=10) and nondiabetic (n=ll) rhesus monkeys. PAI-1 immunoreactivity was significantly increased (PO.001) in retinal capillaries of diabetic monkeys (3.6 fold over nondiabetic controls). In the diabetic monkeys, PAI-1 was seen in the capillary lumen, within the cytoplasm of endothelial cells and in the basement membrane. Minimal PAI-1 was detected in retinas of nondiabetic monkeys. PAI-1 levels correlated with basement membrane thickness in the diabetic monkeys (P0.001). These studies suggest that PAI-1 is overexpressed in retinal capillaries of monkeys with NIDDM. By decreasing extracellular proteolysis, PAI-1 overexpression may contribute to basement membrane thickening observed in diabetic retinopathy.

Original languageEnglish (US)
Pages (from-to)26
Number of pages1
JournalFibrinolysis and Proteolysis
Volume11
Issue numberSUPPL. 3
StatePublished - 1997
Externally publishedYes

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Plasminogen Activator Inhibitor 1
Haplorhini
Basement Membrane
Type 2 Diabetes Mellitus
Proteolysis
Retina
Endothelial Cells
Gold Colloid
Fibrinolysis
Diabetic Retinopathy
Macaca mulatta
Extracellular Matrix
Cytoplasm
Immunohistochemistry
Antigens

ASJC Scopus subject areas

  • Hematology

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Diabetes related increase in plasminogen activator inhibitor-1 expression in monkey retinal capillaries. / Grant, M. B.; Spoerri, P. E.

In: Fibrinolysis and Proteolysis, Vol. 11, No. SUPPL. 3, 1997, p. 26.

Research output: Contribution to journalArticle

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