Diabetic retinopathy is associated with bone marrow neuropathy and a depressed peripheral clock

Julia V. Busik, Maria Tikhonenko, Ashay Bhatwadekar, Madalina Opreanu, Nafissa Yakubova, Sergio Caballero, Danny Player, Takahiko Nakagawa, Aqeela Afzal, Jennifer Kielczewski, Andrew Sochacki, Stephanie Hasty, Sergio Li Calzi, Sungjin Kim, Shane K. Duclas, Mark S. Segal, Dennis L. Guberski, Walter J. Esselman, Michael E. Boulton, Maria B. Grant

Research output: Contribution to journalArticle

154 Citations (Scopus)

Abstract

The present epidemic of diabetes is resulting in a worldwide increase in cardiovascular and microvascular complications including retinopathy. Current thinking has focused on local influences in the retina as being responsible for development of this diabetic complication. However, the contribution of circulating cells in maintenance, repair, and dysfunction of the vasculature is now becoming appreciated. Diabetic individuals have fewer endothelial progenitor cells (EPCs) in their circulation and these cells have diminished migratory potential, which contributes to their decreased reparative capacity. Using a rat model of type 2 diabetes, we show that the decrease in EPC release from diabetic bone marrow is caused by bone marrow neuropathy and that these changes precede the development of diabetic retinopathy. In rats that had diabetes for 4 mo, we observed a dramatic reduction in the number of nerve terminal endings in the bone marrow. Denervation was accompanied by increased numbers of EPCs within the bone marrow but decreased numbers in circulation. Furthermore, denervation was accompanied by a loss of circadian release of EPCs and a marked reduction in clock gene expression in the retina and in EPCs themselves. This reduction in the circadian peak of EPC release led to diminished reparative capacity, resulting in the development of the hallmark feature of diabetic retinopathy, acellular retinal capillaries. Thus, for the first time, diabetic retinopathy is related to neuropathy of the bone marrow. This novel finding shows that bone marrow denervation represents a new therapeutic target for treatment of diabetic vascular complications.

Original languageEnglish (US)
Pages (from-to)2897-2906
Number of pages10
JournalJournal of Experimental Medicine
Volume206
Issue number13
DOIs
StatePublished - Dec 21 2009
Externally publishedYes

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Diabetic Retinopathy
Bone Marrow
Denervation
Retina
Diabetic Angiopathies
Nerve Endings
Diabetes Complications
Type 2 Diabetes Mellitus
Endothelial Progenitor Cells
Maintenance
Gene Expression

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Medicine(all)

Cite this

Busik, J. V., Tikhonenko, M., Bhatwadekar, A., Opreanu, M., Yakubova, N., Caballero, S., ... Grant, M. B. (2009). Diabetic retinopathy is associated with bone marrow neuropathy and a depressed peripheral clock. Journal of Experimental Medicine, 206(13), 2897-2906. https://doi.org/10.1084/jem.20090889

Diabetic retinopathy is associated with bone marrow neuropathy and a depressed peripheral clock. / Busik, Julia V.; Tikhonenko, Maria; Bhatwadekar, Ashay; Opreanu, Madalina; Yakubova, Nafissa; Caballero, Sergio; Player, Danny; Nakagawa, Takahiko; Afzal, Aqeela; Kielczewski, Jennifer; Sochacki, Andrew; Hasty, Stephanie; Li Calzi, Sergio; Kim, Sungjin; Duclas, Shane K.; Segal, Mark S.; Guberski, Dennis L.; Esselman, Walter J.; Boulton, Michael E.; Grant, Maria B.

In: Journal of Experimental Medicine, Vol. 206, No. 13, 21.12.2009, p. 2897-2906.

Research output: Contribution to journalArticle

Busik, JV, Tikhonenko, M, Bhatwadekar, A, Opreanu, M, Yakubova, N, Caballero, S, Player, D, Nakagawa, T, Afzal, A, Kielczewski, J, Sochacki, A, Hasty, S, Li Calzi, S, Kim, S, Duclas, SK, Segal, MS, Guberski, DL, Esselman, WJ, Boulton, ME & Grant, MB 2009, 'Diabetic retinopathy is associated with bone marrow neuropathy and a depressed peripheral clock', Journal of Experimental Medicine, vol. 206, no. 13, pp. 2897-2906. https://doi.org/10.1084/jem.20090889
Busik, Julia V. ; Tikhonenko, Maria ; Bhatwadekar, Ashay ; Opreanu, Madalina ; Yakubova, Nafissa ; Caballero, Sergio ; Player, Danny ; Nakagawa, Takahiko ; Afzal, Aqeela ; Kielczewski, Jennifer ; Sochacki, Andrew ; Hasty, Stephanie ; Li Calzi, Sergio ; Kim, Sungjin ; Duclas, Shane K. ; Segal, Mark S. ; Guberski, Dennis L. ; Esselman, Walter J. ; Boulton, Michael E. ; Grant, Maria B. / Diabetic retinopathy is associated with bone marrow neuropathy and a depressed peripheral clock. In: Journal of Experimental Medicine. 2009 ; Vol. 206, No. 13. pp. 2897-2906.
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