Diagnosis of chronic hepatitis C after liver transplantation by the detection of viral sequences with polymerase chain reaction

John J. Poterucha, Jorge Rakela, Lawrence Lumeng, Chao-Hung Lee, Howard F. Taswell, Russell H. Wiesner

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Abstract

Chronic hepatitis frequently occurs after liver transplantation. The role of hepatitis C virus infection in patients after liver transplantation is unknown, although antibodies to HCV are detected in some of these cases. The use of polymerase chain reaction techniques for the detection of hepatitis C virus RNA should improve sensitivity and specificity, particularly in these immunosuppressed patients. Our goal was to further clarify the role of hepatitis C virus infection in chronic hepatitis occurring after liver transplantation. Patients with chronic hepatitis of uncertain origin after transplantation were identified. Serum samples taken at the time of the most recent liver biopsy that showed chronic hepatitis were tested for anti-hepatitis C virus using enzyme-linked immunoassay and supplemented by recombinant immu-noblot assay (recombinant immunoblot assay I and recombinant immunoblot assay II). The samples were also tested for the presence of hepatitis C virus RNA using polymerase chain reaction. Of the 25 patients with chronic hepatitis, 15 (60%) had hepatitis C virus RNA present. Only seven (47%) of these 15 patients had anti-hepatitis C virus detected. Hepatitis C virus is a major cause of chronic hepatitis occurring after liver transplantation. The magnitude of hepatitis C virus infection will be underestimated if only currently available assays for anti-hepatitis C virus are used.

Original languageEnglish
Pages (from-to)42-45
Number of pages4
JournalHepatology
Volume15
Issue number1
StatePublished - Jan 1992

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Chronic Hepatitis C
Hepacivirus
Liver Transplantation
Chronic Hepatitis
Polymerase Chain Reaction
Virus Diseases
RNA
Hepatitis C Antibodies
DNA-Directed RNA Polymerases
Immunoenzyme Techniques
Transplantation
Biopsy
Sensitivity and Specificity
Liver

ASJC Scopus subject areas

  • Hepatology

Cite this

Poterucha, J. J., Rakela, J., Lumeng, L., Lee, C-H., Taswell, H. F., & Wiesner, R. H. (1992). Diagnosis of chronic hepatitis C after liver transplantation by the detection of viral sequences with polymerase chain reaction. Hepatology, 15(1), 42-45.

Diagnosis of chronic hepatitis C after liver transplantation by the detection of viral sequences with polymerase chain reaction. / Poterucha, John J.; Rakela, Jorge; Lumeng, Lawrence; Lee, Chao-Hung; Taswell, Howard F.; Wiesner, Russell H.

In: Hepatology, Vol. 15, No. 1, 01.1992, p. 42-45.

Research output: Contribution to journalArticle

Poterucha, JJ, Rakela, J, Lumeng, L, Lee, C-H, Taswell, HF & Wiesner, RH 1992, 'Diagnosis of chronic hepatitis C after liver transplantation by the detection of viral sequences with polymerase chain reaction', Hepatology, vol. 15, no. 1, pp. 42-45.
Poterucha, John J. ; Rakela, Jorge ; Lumeng, Lawrence ; Lee, Chao-Hung ; Taswell, Howard F. ; Wiesner, Russell H. / Diagnosis of chronic hepatitis C after liver transplantation by the detection of viral sequences with polymerase chain reaction. In: Hepatology. 1992 ; Vol. 15, No. 1. pp. 42-45.
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