Diagnosis of pneumocystis carinii pneumonia in aids patients by using polymerase chain reactions on serum specimens

Chiara Atzori, Jang Jih Lu, Bingdong Jiang, Marilyn S. Bartlett, Giovanna Orlando, Sherry F. Queener, James W. Smith, Antonietta Cargnel, Chao Hung Lee

Research output: Contribution to journalArticlepeer-review

38 Scopus citations

Abstract

A polymerase chain reaction (PCR) that amplifies the internal transcribed spacers of rRNA genes of Pneumocystis carinii (Pc-ITS-PCR) was compared with dihydrofolate reductase (DHFR) gene PCR to determine its usefulness in the diagnosis of Pneumocystis carinii pneumonia. All specimens from non-P. carinii pneumonia AIDS patients were both Pc-ITS-PCR- and DHFR PCR-negative. Twenty-six (96%) of 27 bronchoalveolar lavage specimens from P. carinii pneumonia patients were Pc-ITS-PCR-positive, and all 27 of these patients had at least 1 serum sample positive by Pc-ITS-PCR. In contrast, DHFR PCR detected P. carinii in only 4 (17%) of 24 P. carinii-positive lavage specimens and 2 (10%) of the 20 sera from P. carinii pneumonia patients. These results suggest that serum Pc-ITS-PCR may be useful for the diagnosis of P. carinii pneumonia.

Original languageEnglish (US)
Pages (from-to)1623-1626
Number of pages4
JournalJournal of Infectious Diseases
Volume172
Issue number6
DOIs
StatePublished - Dec 1995

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

Fingerprint Dive into the research topics of 'Diagnosis of pneumocystis carinii pneumonia in aids patients by using polymerase chain reactions on serum specimens'. Together they form a unique fingerprint.

Cite this