Diagnosis of Pneumocystis carinii pneumonia in AIDS patients by using polymerase chain reactions on serum specimens

Chiara Atzori, Jang Jih Lu, Bingdong Jiang, Marilyn S. Bartlett, Giovanna Orlando, Sherry Queener, James W. Smith, Antonietta Cargnel, Chao-Hung Lee

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

A polymerase chain reaction (PCR) that amplifies the internal transcribed spacers of rRNA genes of Pneumocystis carinii (Pc-ITS-PCR) was compared with dihydrofolate reductase (DHFR) gene PCR to determine its usefulness in the diagnosis of Pneumocystis carinii pneumonia. All specimens from non-P. carinii pneumonia AIDS patients were both Pc-ITS-PCR- and DHFR PCR-negative. Twenty-six (96%) of 27 bronchoalveolar lavage specimens from P. carinii pneumonia patients were Pc-ITS-PCR-positive, and all 27 of these patients had at least 1 serum sample positive by Pc-ITS-PCR. In contrast, DHFR PCR detected P. carinii in only 4 (17%) of 24 P. carinii-positive lavage specimens and 2 (10%) of the 20 sera from P. carinii pneumonia patients. These results suggest that serum Pc-ITS-PCR may be useful for the diagnosis of P. carinii pneumonia.

Original languageEnglish
Pages (from-to)1623-1626
Number of pages4
JournalJournal of Infectious Diseases
Volume172
Issue number6
StatePublished - Dec 1995

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Pneumocystis Pneumonia
Acquired Immunodeficiency Syndrome
Polymerase Chain Reaction
Serum
Pneumocystis carinii
Tetrahydrofolate Dehydrogenase
Therapeutic Irrigation
Bronchoalveolar Lavage
rRNA Genes
Pneumonia

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Immunology

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Diagnosis of Pneumocystis carinii pneumonia in AIDS patients by using polymerase chain reactions on serum specimens. / Atzori, Chiara; Lu, Jang Jih; Jiang, Bingdong; Bartlett, Marilyn S.; Orlando, Giovanna; Queener, Sherry; Smith, James W.; Cargnel, Antonietta; Lee, Chao-Hung.

In: Journal of Infectious Diseases, Vol. 172, No. 6, 12.1995, p. 1623-1626.

Research output: Contribution to journalArticle

Atzori, C, Lu, JJ, Jiang, B, Bartlett, MS, Orlando, G, Queener, S, Smith, JW, Cargnel, A & Lee, C-H 1995, 'Diagnosis of Pneumocystis carinii pneumonia in AIDS patients by using polymerase chain reactions on serum specimens', Journal of Infectious Diseases, vol. 172, no. 6, pp. 1623-1626.
Atzori, Chiara ; Lu, Jang Jih ; Jiang, Bingdong ; Bartlett, Marilyn S. ; Orlando, Giovanna ; Queener, Sherry ; Smith, James W. ; Cargnel, Antonietta ; Lee, Chao-Hung. / Diagnosis of Pneumocystis carinii pneumonia in AIDS patients by using polymerase chain reactions on serum specimens. In: Journal of Infectious Diseases. 1995 ; Vol. 172, No. 6. pp. 1623-1626.
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