Diagnosis of pulmonary histoplasmosis and blastomycosis by detection of antigen in bronchoalveolar lavage fluid using an improved second-generation enzyme-linked immunoassay

Chadi Hage, Thomas Davis, Lindsey Egan, Michelle Parker, Deanna Fuller, Ann M. LeMonte, Michelle Durkin, Patricia Connelly, L. Joseph Wheat, Deborah Blue-Hnidy, Kenneth S. Knox

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Antigen detection is a useful adjunct for the diagnosis of histoplasmosis. The purpose of this study was to evaluate antigen detection in bronchoalveolar lavage (BAL) fluid using an improved second-generation Histoplasma antigen assay. Antigen was detected in 16 of 19 (84%) cases of histoplasmosis and 5 of 6 (83.3%) blastomycosis cases using the second-generation assay vs. 13 of 19 (68%) and 4 of 6 (66.7%), respectively, in the original assay. Ten-fold concentration permitted detection of antigen in an additional case of histoplasmosis and another with blastomycosis, for an overall sensitivity of 23 of 25 (92.0%). Specificity was 98.2% in both assays in controls with other pulmonary infections. These findings support the diagnostic utility of the second-generation assay in patients with pulmonary histoplasmosis and blastomycosis.

Original languageEnglish
Pages (from-to)43-47
Number of pages5
JournalRespiratory Medicine
Volume101
Issue number1
DOIs
StatePublished - Jan 2007

Fingerprint

Blastomycosis
Histoplasmosis
Bronchoalveolar Lavage Fluid
Immunoenzyme Techniques
Antigens
Lung
Histoplasma
Infection

Keywords

  • Blastomycosis
  • Bronchoalveolar lavage
  • Histoplasma antigen
  • Histoplasmosis
  • Second-generation assay

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

Cite this

Diagnosis of pulmonary histoplasmosis and blastomycosis by detection of antigen in bronchoalveolar lavage fluid using an improved second-generation enzyme-linked immunoassay. / Hage, Chadi; Davis, Thomas; Egan, Lindsey; Parker, Michelle; Fuller, Deanna; LeMonte, Ann M.; Durkin, Michelle; Connelly, Patricia; Joseph Wheat, L.; Blue-Hnidy, Deborah; Knox, Kenneth S.

In: Respiratory Medicine, Vol. 101, No. 1, 01.2007, p. 43-47.

Research output: Contribution to journalArticle

Hage, Chadi ; Davis, Thomas ; Egan, Lindsey ; Parker, Michelle ; Fuller, Deanna ; LeMonte, Ann M. ; Durkin, Michelle ; Connelly, Patricia ; Joseph Wheat, L. ; Blue-Hnidy, Deborah ; Knox, Kenneth S. / Diagnosis of pulmonary histoplasmosis and blastomycosis by detection of antigen in bronchoalveolar lavage fluid using an improved second-generation enzyme-linked immunoassay. In: Respiratory Medicine. 2007 ; Vol. 101, No. 1. pp. 43-47.
@article{9428901751b840a1a302e6fb2ca900d8,
title = "Diagnosis of pulmonary histoplasmosis and blastomycosis by detection of antigen in bronchoalveolar lavage fluid using an improved second-generation enzyme-linked immunoassay",
abstract = "Antigen detection is a useful adjunct for the diagnosis of histoplasmosis. The purpose of this study was to evaluate antigen detection in bronchoalveolar lavage (BAL) fluid using an improved second-generation Histoplasma antigen assay. Antigen was detected in 16 of 19 (84{\%}) cases of histoplasmosis and 5 of 6 (83.3{\%}) blastomycosis cases using the second-generation assay vs. 13 of 19 (68{\%}) and 4 of 6 (66.7{\%}), respectively, in the original assay. Ten-fold concentration permitted detection of antigen in an additional case of histoplasmosis and another with blastomycosis, for an overall sensitivity of 23 of 25 (92.0{\%}). Specificity was 98.2{\%} in both assays in controls with other pulmonary infections. These findings support the diagnostic utility of the second-generation assay in patients with pulmonary histoplasmosis and blastomycosis.",
keywords = "Blastomycosis, Bronchoalveolar lavage, Histoplasma antigen, Histoplasmosis, Second-generation assay",
author = "Chadi Hage and Thomas Davis and Lindsey Egan and Michelle Parker and Deanna Fuller and LeMonte, {Ann M.} and Michelle Durkin and Patricia Connelly and {Joseph Wheat}, L. and Deborah Blue-Hnidy and Knox, {Kenneth S.}",
year = "2007",
month = "1",
doi = "10.1016/j.rmed.2006.04.017",
language = "English",
volume = "101",
pages = "43--47",
journal = "Respiratory Medicine",
issn = "0954-6111",
publisher = "W.B. Saunders Ltd",
number = "1",

}

TY - JOUR

T1 - Diagnosis of pulmonary histoplasmosis and blastomycosis by detection of antigen in bronchoalveolar lavage fluid using an improved second-generation enzyme-linked immunoassay

AU - Hage, Chadi

AU - Davis, Thomas

AU - Egan, Lindsey

AU - Parker, Michelle

AU - Fuller, Deanna

AU - LeMonte, Ann M.

AU - Durkin, Michelle

AU - Connelly, Patricia

AU - Joseph Wheat, L.

AU - Blue-Hnidy, Deborah

AU - Knox, Kenneth S.

PY - 2007/1

Y1 - 2007/1

N2 - Antigen detection is a useful adjunct for the diagnosis of histoplasmosis. The purpose of this study was to evaluate antigen detection in bronchoalveolar lavage (BAL) fluid using an improved second-generation Histoplasma antigen assay. Antigen was detected in 16 of 19 (84%) cases of histoplasmosis and 5 of 6 (83.3%) blastomycosis cases using the second-generation assay vs. 13 of 19 (68%) and 4 of 6 (66.7%), respectively, in the original assay. Ten-fold concentration permitted detection of antigen in an additional case of histoplasmosis and another with blastomycosis, for an overall sensitivity of 23 of 25 (92.0%). Specificity was 98.2% in both assays in controls with other pulmonary infections. These findings support the diagnostic utility of the second-generation assay in patients with pulmonary histoplasmosis and blastomycosis.

AB - Antigen detection is a useful adjunct for the diagnosis of histoplasmosis. The purpose of this study was to evaluate antigen detection in bronchoalveolar lavage (BAL) fluid using an improved second-generation Histoplasma antigen assay. Antigen was detected in 16 of 19 (84%) cases of histoplasmosis and 5 of 6 (83.3%) blastomycosis cases using the second-generation assay vs. 13 of 19 (68%) and 4 of 6 (66.7%), respectively, in the original assay. Ten-fold concentration permitted detection of antigen in an additional case of histoplasmosis and another with blastomycosis, for an overall sensitivity of 23 of 25 (92.0%). Specificity was 98.2% in both assays in controls with other pulmonary infections. These findings support the diagnostic utility of the second-generation assay in patients with pulmonary histoplasmosis and blastomycosis.

KW - Blastomycosis

KW - Bronchoalveolar lavage

KW - Histoplasma antigen

KW - Histoplasmosis

KW - Second-generation assay

UR - http://www.scopus.com/inward/record.url?scp=33751531602&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33751531602&partnerID=8YFLogxK

U2 - 10.1016/j.rmed.2006.04.017

DO - 10.1016/j.rmed.2006.04.017

M3 - Article

C2 - 16753290

AN - SCOPUS:33751531602

VL - 101

SP - 43

EP - 47

JO - Respiratory Medicine

JF - Respiratory Medicine

SN - 0954-6111

IS - 1

ER -