Diagnostic immunohistochemistry of canine round cell tumors.

George Sandusky, W. W. Carlton, K. A. Wightman

Research output: Contribution to journalArticle

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Abstract

Sixty-five canine skin neoplasms studied using immunocytochemistry, included 22 histiocytomas, 18 amelanotic melanomas, 14 cutaneous lymphosarcomas, six mast cell tumors, and five transmissible venereal tumors. Formalin-fixed, paraffin-embedded sections were stained using the avidin-biotin-peroxidase complex (ABC) immunoperoxidase technique for reactivity with S-100 protein, kappa and lambda immunoglobulin light chains, alpha-1-antitrypsin, alpha-1-antichymotrypsin, leukocyte common antigen (LCA), neuron-specific enolase, keratin, cytokeratin, muramidase, and vimentin. Detection of S-100, kappa and lambda light chains, neuron-specific enolase, and vimentin were most useful for screening these neoplasms. None of the markers examined was consistent in staining histiocytomas. While reactivity of S-100 (ten cases) and neuron-specific enolase (ten cases) was detected in some amelanotic melanomas, lambda light chain immunoglobulin (eight cases) was relatively consistent in cutaneous lymphomas. Mast cell neoplasms reacted with avidin and, therefore, were positive, even on negative control sections. Vimentin reacted strongly on all amelanotic melanomas and transmissible venereal tumors examined. These antibodies are helpful adjuncts in the differential diagnosis of canine skin tumors.

Original languageEnglish (US)
Pages (from-to)495-499
Number of pages5
JournalVeterinary Pathology
Volume24
Issue number6
StatePublished - Nov 1987
Externally publishedYes

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phosphopyruvate hydratase
vimentin
Amelanotic Melanoma
melanoma
immunoglobulin light chains
transmissible venereal tumors
immunohistochemistry
Phosphopyruvate Hydratase
Canidae
Veterinary Venereal Tumors
Immunoglobulin lambda-Chains
avidin
Vimentin
Histiocytoma
neurons
Immunohistochemistry
mast cells
neoplasms
Avidin
dogs

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Sandusky, G., Carlton, W. W., & Wightman, K. A. (1987). Diagnostic immunohistochemistry of canine round cell tumors. Veterinary Pathology, 24(6), 495-499.

Diagnostic immunohistochemistry of canine round cell tumors. / Sandusky, George; Carlton, W. W.; Wightman, K. A.

In: Veterinary Pathology, Vol. 24, No. 6, 11.1987, p. 495-499.

Research output: Contribution to journalArticle

Sandusky, G, Carlton, WW & Wightman, KA 1987, 'Diagnostic immunohistochemistry of canine round cell tumors.', Veterinary Pathology, vol. 24, no. 6, pp. 495-499.
Sandusky, George ; Carlton, W. W. ; Wightman, K. A. / Diagnostic immunohistochemistry of canine round cell tumors. In: Veterinary Pathology. 1987 ; Vol. 24, No. 6. pp. 495-499.
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