Diagnostic usefulness of fluorescent cytogenetics in differentiating chromophobe renal cell carcinoma from renal oncocytoma: A validation study combining metaphase and interphase analyses

Matteo Brunelli, Brett Delahunt, Stefano Gobbo, Regina Tardanico, Albino Eccher, Samantha Bersani, Paolo Cossu-Rocca, Claudia Parolini, Piera Balzarini, Fabio Menestrina, Liang Cheng, John Eble, Guido Martignoni

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

We investigated the usefulness of interphase fluorescence in situ hybridization (FISH) analysis to differentiate between 11 chromophobe renal carcinomas and 12 renal oncocytomas, showing different clinical outcomes, when compared with conventional metaphase cytogenetics by karyotyping. Karyotypically, 3 chromophobe renal cell carcinomas showed losses of chromosomes, 3 were polyploid, 1 was normal, and 4 failed to grow. Of 12 oncocytomas, 5 showed a normal numeric karyotype and 6 additional structural rearrangements. FISH on chromophobe renal cell carcinomas showed a high percentage of cases (10/11 [91%]) with multiple numeric losses among chromosomes 1, 2, 6, 10, and 17; this interphase pattern was observed irrespective of the 3 different metaphase karyotypes. Of 12 oncocytomas, 11 (92%) revealed a normal numeric chromosomal status showing at least 2 chromosomes without aneusomy by interphase FISH. The study demonstrates that indeed FISH performed on formalin-fixed, paraffin-embedded tissue can provide clinically useful information more reliably than karyotyping of most of these tumors.

Original languageEnglish
Pages (from-to)116-126
Number of pages11
JournalAmerican Journal of Clinical Pathology
Volume133
Issue number1
DOIs
StatePublished - Jan 2010

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Validation Studies
Interphase
Metaphase
Fluorescence In Situ Hybridization
Renal Cell Carcinoma
Cytogenetics
Oxyphilic Adenoma
Karyotyping
Chromosomes, Human, Pair 2
Karyotype
Chromosomes, Human, Pair 3
Polyploidy
Chromosomes, Human, Pair 1
Paraffin
Formaldehyde
Carcinoma
Kidney
Renal oncocytoma
Neoplasms

Keywords

  • Chromophobe renal carcinoma
  • Interphase fluorescence in situ hybridization
  • Metaphase karyotyping
  • Oncocytoma

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Diagnostic usefulness of fluorescent cytogenetics in differentiating chromophobe renal cell carcinoma from renal oncocytoma : A validation study combining metaphase and interphase analyses. / Brunelli, Matteo; Delahunt, Brett; Gobbo, Stefano; Tardanico, Regina; Eccher, Albino; Bersani, Samantha; Cossu-Rocca, Paolo; Parolini, Claudia; Balzarini, Piera; Menestrina, Fabio; Cheng, Liang; Eble, John; Martignoni, Guido.

In: American Journal of Clinical Pathology, Vol. 133, No. 1, 01.2010, p. 116-126.

Research output: Contribution to journalArticle

Brunelli, Matteo ; Delahunt, Brett ; Gobbo, Stefano ; Tardanico, Regina ; Eccher, Albino ; Bersani, Samantha ; Cossu-Rocca, Paolo ; Parolini, Claudia ; Balzarini, Piera ; Menestrina, Fabio ; Cheng, Liang ; Eble, John ; Martignoni, Guido. / Diagnostic usefulness of fluorescent cytogenetics in differentiating chromophobe renal cell carcinoma from renal oncocytoma : A validation study combining metaphase and interphase analyses. In: American Journal of Clinical Pathology. 2010 ; Vol. 133, No. 1. pp. 116-126.
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