Diagnostic usefulness of fluorescent cytogenetics in differentiating chromophobe renal cell carcinoma from renal oncocytoma: A validation study combining metaphase and interphase analyses

Matteo Brunelli, Brett Delahunt, Stefano Gobbo, Regina Tardanico, Albino Eccher, Samantha Bersani, Paolo Cossu-Rocca, Claudia Parolini, Piera Balzarini, Fabio Menestrina, Liang Cheng, John Nelson Eble, Guido Martignoni

Research output: Contribution to journalArticle

25 Scopus citations

Abstract

We investigated the usefulness of interphase fluorescence in situ hybridization (FISH) analysis to differentiate between 11 chromophobe renal carcinomas and 12 renal oncocytomas, showing different clinical outcomes, when compared with conventional metaphase cytogenetics by karyotyping. Karyotypically, 3 chromophobe renal cell carcinomas showed losses of chromosomes, 3 were polyploid, 1 was normal, and 4 failed to grow. Of 12 oncocytomas, 5 showed a normal numeric karyotype and 6 additional structural rearrangements. FISH on chromophobe renal cell carcinomas showed a high percentage of cases (10/11 [91%]) with multiple numeric losses among chromosomes 1, 2, 6, 10, and 17; this interphase pattern was observed irrespective of the 3 different metaphase karyotypes. Of 12 oncocytomas, 11 (92%) revealed a normal numeric chromosomal status showing at least 2 chromosomes without aneusomy by interphase FISH. The study demonstrates that indeed FISH performed on formalin-fixed, paraffin-embedded tissue can provide clinically useful information more reliably than karyotyping of most of these tumors.

Original languageEnglish (US)
Pages (from-to)116-126
Number of pages11
JournalAmerican journal of clinical pathology
Volume133
Issue number1
DOIs
StatePublished - Jan 1 2010

Keywords

  • Chromophobe renal carcinoma
  • Interphase fluorescence in situ hybridization
  • Metaphase karyotyping
  • Oncocytoma

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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