Diagnostic utility of S100A1 expression in renal cell neoplasms: An immunohistochemical and quantitative RT-PCR study

Paolo Cossu Rocca, Matteo Brunelli, Stefano Gobbo, Albino Eccher, Emma Bragantini, Maria M. Mina, Vincenzo Ficarra, Filiberto Zattoni, Alberto Zamò, Maurizio Pea, Aldo Scarpa, Marco Chilosi, Fabio Menestrina, Franco Bonetti, John Eble, Guido Martignoni

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

S100A1 is a calcium-binding protein, which has been recently found in renal cell neoplasms. We evaluated the diagnostic utility of immunohistochemical detection of S100A1 in 164 renal cell neoplasms. Forty-one clear cell, 32 papillary, and 51 chromophobe renal cell carcinomas, and 40 oncocytomas, 164 samples of normal renal parenchyma adjacent to the tumors and 13 fetal kidneys were analyzed. The levels of S100A1 mRNA detected by quantitative RT-PCR analysis of frozen tissues from seven clear cell, five papillary, and six chromophobe renal cell carcinomas, four oncocytomas, and nine samples of normal renal tissues adjacent to neoplasms were compared with the immunohistochemical detection of protein expression. Clear cell and papillary renal cell carcinomas showed positive reactions for S100A1 in 30 out of 41 tumors (73%) and in 30 out of 32 (94%) tumors, respectively. Thirty-seven renal oncocytomas out of 40 (93%) were positive for S100A1, whereas 48 of 51 (94%) chromophobe renal cell carcinomas were negative. S100A1 protein was detected in all samples of unaffected and fetal kidneys. S100A1 mRNA was detected by RT-PCR in all normal kidneys and renal cell neoplasms, although at very different levels. Statistical analyses comparing the different expression of S100A1 in clear cell and chromophobe renal cell carcinomas observed by immunohistochemical and RT-PCR methods showed significant values (P<0.001), such as when comparing by both techniques the different levels of S100A1 expression in chromophobe renal cell carcinomas and oncocytomas (P<0.001). Our study shows that S100A1 protein is expressed in oncocytomas, clear cell and papillary renal cell carcinomas but not in chromophobe renal cell carcinomas. Its immunodetection is potentially useful for the differential diagnosis between chromophobe renal cell carcinoma and oncocytoma. Further, S100A1 protein expression is constantly detected in the normal parenchyma of the adult and fetal kidney.

Original languageEnglish
Pages (from-to)722-728
Number of pages7
JournalModern Pathology
Volume20
Issue number7
DOIs
StatePublished - Jul 2007

Fingerprint

Kidney Neoplasms
Renal Cell Carcinoma
Polymerase Chain Reaction
Oxyphilic Adenoma
Kidney
Neoplasms
Messenger RNA
Calcium-Binding Proteins
Differential Diagnosis

Keywords

  • Chromophobe renal cell carcinoma
  • Oncocytoma
  • S100A1

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Rocca, P. C., Brunelli, M., Gobbo, S., Eccher, A., Bragantini, E., Mina, M. M., ... Martignoni, G. (2007). Diagnostic utility of S100A1 expression in renal cell neoplasms: An immunohistochemical and quantitative RT-PCR study. Modern Pathology, 20(7), 722-728. https://doi.org/10.1038/modpathol.3800828

Diagnostic utility of S100A1 expression in renal cell neoplasms : An immunohistochemical and quantitative RT-PCR study. / Rocca, Paolo Cossu; Brunelli, Matteo; Gobbo, Stefano; Eccher, Albino; Bragantini, Emma; Mina, Maria M.; Ficarra, Vincenzo; Zattoni, Filiberto; Zamò, Alberto; Pea, Maurizio; Scarpa, Aldo; Chilosi, Marco; Menestrina, Fabio; Bonetti, Franco; Eble, John; Martignoni, Guido.

In: Modern Pathology, Vol. 20, No. 7, 07.2007, p. 722-728.

Research output: Contribution to journalArticle

Rocca, PC, Brunelli, M, Gobbo, S, Eccher, A, Bragantini, E, Mina, MM, Ficarra, V, Zattoni, F, Zamò, A, Pea, M, Scarpa, A, Chilosi, M, Menestrina, F, Bonetti, F, Eble, J & Martignoni, G 2007, 'Diagnostic utility of S100A1 expression in renal cell neoplasms: An immunohistochemical and quantitative RT-PCR study', Modern Pathology, vol. 20, no. 7, pp. 722-728. https://doi.org/10.1038/modpathol.3800828
Rocca, Paolo Cossu ; Brunelli, Matteo ; Gobbo, Stefano ; Eccher, Albino ; Bragantini, Emma ; Mina, Maria M. ; Ficarra, Vincenzo ; Zattoni, Filiberto ; Zamò, Alberto ; Pea, Maurizio ; Scarpa, Aldo ; Chilosi, Marco ; Menestrina, Fabio ; Bonetti, Franco ; Eble, John ; Martignoni, Guido. / Diagnostic utility of S100A1 expression in renal cell neoplasms : An immunohistochemical and quantitative RT-PCR study. In: Modern Pathology. 2007 ; Vol. 20, No. 7. pp. 722-728.
@article{70290ee352be40e5be08d0775d64267c,
title = "Diagnostic utility of S100A1 expression in renal cell neoplasms: An immunohistochemical and quantitative RT-PCR study",
abstract = "S100A1 is a calcium-binding protein, which has been recently found in renal cell neoplasms. We evaluated the diagnostic utility of immunohistochemical detection of S100A1 in 164 renal cell neoplasms. Forty-one clear cell, 32 papillary, and 51 chromophobe renal cell carcinomas, and 40 oncocytomas, 164 samples of normal renal parenchyma adjacent to the tumors and 13 fetal kidneys were analyzed. The levels of S100A1 mRNA detected by quantitative RT-PCR analysis of frozen tissues from seven clear cell, five papillary, and six chromophobe renal cell carcinomas, four oncocytomas, and nine samples of normal renal tissues adjacent to neoplasms were compared with the immunohistochemical detection of protein expression. Clear cell and papillary renal cell carcinomas showed positive reactions for S100A1 in 30 out of 41 tumors (73{\%}) and in 30 out of 32 (94{\%}) tumors, respectively. Thirty-seven renal oncocytomas out of 40 (93{\%}) were positive for S100A1, whereas 48 of 51 (94{\%}) chromophobe renal cell carcinomas were negative. S100A1 protein was detected in all samples of unaffected and fetal kidneys. S100A1 mRNA was detected by RT-PCR in all normal kidneys and renal cell neoplasms, although at very different levels. Statistical analyses comparing the different expression of S100A1 in clear cell and chromophobe renal cell carcinomas observed by immunohistochemical and RT-PCR methods showed significant values (P<0.001), such as when comparing by both techniques the different levels of S100A1 expression in chromophobe renal cell carcinomas and oncocytomas (P<0.001). Our study shows that S100A1 protein is expressed in oncocytomas, clear cell and papillary renal cell carcinomas but not in chromophobe renal cell carcinomas. Its immunodetection is potentially useful for the differential diagnosis between chromophobe renal cell carcinoma and oncocytoma. Further, S100A1 protein expression is constantly detected in the normal parenchyma of the adult and fetal kidney.",
keywords = "Chromophobe renal cell carcinoma, Oncocytoma, S100A1",
author = "Rocca, {Paolo Cossu} and Matteo Brunelli and Stefano Gobbo and Albino Eccher and Emma Bragantini and Mina, {Maria M.} and Vincenzo Ficarra and Filiberto Zattoni and Alberto Zam{\`o} and Maurizio Pea and Aldo Scarpa and Marco Chilosi and Fabio Menestrina and Franco Bonetti and John Eble and Guido Martignoni",
year = "2007",
month = "7",
doi = "10.1038/modpathol.3800828",
language = "English",
volume = "20",
pages = "722--728",
journal = "Modern Pathology",
issn = "0893-3952",
publisher = "Nature Publishing Group",
number = "7",

}

TY - JOUR

T1 - Diagnostic utility of S100A1 expression in renal cell neoplasms

T2 - An immunohistochemical and quantitative RT-PCR study

AU - Rocca, Paolo Cossu

AU - Brunelli, Matteo

AU - Gobbo, Stefano

AU - Eccher, Albino

AU - Bragantini, Emma

AU - Mina, Maria M.

AU - Ficarra, Vincenzo

AU - Zattoni, Filiberto

AU - Zamò, Alberto

AU - Pea, Maurizio

AU - Scarpa, Aldo

AU - Chilosi, Marco

AU - Menestrina, Fabio

AU - Bonetti, Franco

AU - Eble, John

AU - Martignoni, Guido

PY - 2007/7

Y1 - 2007/7

N2 - S100A1 is a calcium-binding protein, which has been recently found in renal cell neoplasms. We evaluated the diagnostic utility of immunohistochemical detection of S100A1 in 164 renal cell neoplasms. Forty-one clear cell, 32 papillary, and 51 chromophobe renal cell carcinomas, and 40 oncocytomas, 164 samples of normal renal parenchyma adjacent to the tumors and 13 fetal kidneys were analyzed. The levels of S100A1 mRNA detected by quantitative RT-PCR analysis of frozen tissues from seven clear cell, five papillary, and six chromophobe renal cell carcinomas, four oncocytomas, and nine samples of normal renal tissues adjacent to neoplasms were compared with the immunohistochemical detection of protein expression. Clear cell and papillary renal cell carcinomas showed positive reactions for S100A1 in 30 out of 41 tumors (73%) and in 30 out of 32 (94%) tumors, respectively. Thirty-seven renal oncocytomas out of 40 (93%) were positive for S100A1, whereas 48 of 51 (94%) chromophobe renal cell carcinomas were negative. S100A1 protein was detected in all samples of unaffected and fetal kidneys. S100A1 mRNA was detected by RT-PCR in all normal kidneys and renal cell neoplasms, although at very different levels. Statistical analyses comparing the different expression of S100A1 in clear cell and chromophobe renal cell carcinomas observed by immunohistochemical and RT-PCR methods showed significant values (P<0.001), such as when comparing by both techniques the different levels of S100A1 expression in chromophobe renal cell carcinomas and oncocytomas (P<0.001). Our study shows that S100A1 protein is expressed in oncocytomas, clear cell and papillary renal cell carcinomas but not in chromophobe renal cell carcinomas. Its immunodetection is potentially useful for the differential diagnosis between chromophobe renal cell carcinoma and oncocytoma. Further, S100A1 protein expression is constantly detected in the normal parenchyma of the adult and fetal kidney.

AB - S100A1 is a calcium-binding protein, which has been recently found in renal cell neoplasms. We evaluated the diagnostic utility of immunohistochemical detection of S100A1 in 164 renal cell neoplasms. Forty-one clear cell, 32 papillary, and 51 chromophobe renal cell carcinomas, and 40 oncocytomas, 164 samples of normal renal parenchyma adjacent to the tumors and 13 fetal kidneys were analyzed. The levels of S100A1 mRNA detected by quantitative RT-PCR analysis of frozen tissues from seven clear cell, five papillary, and six chromophobe renal cell carcinomas, four oncocytomas, and nine samples of normal renal tissues adjacent to neoplasms were compared with the immunohistochemical detection of protein expression. Clear cell and papillary renal cell carcinomas showed positive reactions for S100A1 in 30 out of 41 tumors (73%) and in 30 out of 32 (94%) tumors, respectively. Thirty-seven renal oncocytomas out of 40 (93%) were positive for S100A1, whereas 48 of 51 (94%) chromophobe renal cell carcinomas were negative. S100A1 protein was detected in all samples of unaffected and fetal kidneys. S100A1 mRNA was detected by RT-PCR in all normal kidneys and renal cell neoplasms, although at very different levels. Statistical analyses comparing the different expression of S100A1 in clear cell and chromophobe renal cell carcinomas observed by immunohistochemical and RT-PCR methods showed significant values (P<0.001), such as when comparing by both techniques the different levels of S100A1 expression in chromophobe renal cell carcinomas and oncocytomas (P<0.001). Our study shows that S100A1 protein is expressed in oncocytomas, clear cell and papillary renal cell carcinomas but not in chromophobe renal cell carcinomas. Its immunodetection is potentially useful for the differential diagnosis between chromophobe renal cell carcinoma and oncocytoma. Further, S100A1 protein expression is constantly detected in the normal parenchyma of the adult and fetal kidney.

KW - Chromophobe renal cell carcinoma

KW - Oncocytoma

KW - S100A1

UR - http://www.scopus.com/inward/record.url?scp=34250647837&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34250647837&partnerID=8YFLogxK

U2 - 10.1038/modpathol.3800828

DO - 10.1038/modpathol.3800828

M3 - Article

C2 - 17483815

AN - SCOPUS:34250647837

VL - 20

SP - 722

EP - 728

JO - Modern Pathology

JF - Modern Pathology

SN - 0893-3952

IS - 7

ER -