Diet effects and ontogeny of alterations of circulating insulin-like growth factor binding proteins in newborn dairy calves.

Todd Skaar, C. R. Baumrucker, D. R. Deaver, J. W. Blum

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Insulin-like growth factors (IGF) are important growth regulators in many species, and their effects are influenced by their association with IGF-binding proteins (IGFBP). The objectives of this study were to characterize the ontogeny of the blood plasma IGFBP in calves and to determine the effect of dietary IGF-I neonatal plasma IGFBP. Plasma from newborn and 7-d-old male calves fed milk replacer, milk replacer + recombinant human IGF-I (rhIGF-I), or colostrum for 2 d followed by milk replacer was analyzed for IGFBP by ligand blot analysis. In addition, plasma samples from 1-, 12-, 24-, and 45-wk-old male calves were analyzed for IGFBP and IGF-I. Newborn and 7-d-old calf plasma contained IGFBP with M(r) of 26, 34, and 42 to 48 kDa. These profiles were not affected by the dietary treatments; however, a slight increase in the 34-kDa IGFBP and a slight decrease in the 26- and 42- to 48-kDa IGFBP were detected from birth to 7 d of age. The 34-kDa was confirmed to be bovine IGFBP-2 by immunoblot and the 42- to 48-kDa is likely IGFBP-3. The 29-, 31-, and 42- to 48-kDa IGFBP increased between 1 and 45 wk of age. Similarly, plasma IGF-I concentrations were increased from 49.7 to 449.7 ng/mL in plasma from calves from 1 to 45 wk of age. In contrast, the 34-kDa IGFBP increased from 1 to 12 wk but then gradually decreased from 12 to 45 wk, whereas the 26-kDa IGFBP did not change.(ABSTRACT TRUNCATED AT 250 WORDS)

Original languageEnglish (US)
Pages (from-to)421-427
Number of pages7
JournalJournal of Animal Science
Volume72
Issue number2
StatePublished - Feb 1994
Externally publishedYes

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Insulin-Like Growth Factor Binding Proteins
insulin-like growth factor binding proteins
dairy calves
ontogeny
neonates
Diet
diet
Insulin-Like Growth Factor I
insulin-like growth factor I
milk replacer
calves
Milk
blood proteins
Blood Proteins
Insulin-Like Growth Factor Binding Protein 2
Insulin-Like Growth Factor Binding Protein 1
Insulin-Like Growth Factor Binding Protein 3
Colostrum
Somatomedins
somatomedins

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

Diet effects and ontogeny of alterations of circulating insulin-like growth factor binding proteins in newborn dairy calves. / Skaar, Todd; Baumrucker, C. R.; Deaver, D. R.; Blum, J. W.

In: Journal of Animal Science, Vol. 72, No. 2, 02.1994, p. 421-427.

Research output: Contribution to journalArticle

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