Dietary antioxidants provide differential subcellular protection in epithelial cells

Stuart G. Jarrett, Joy Cuenco, Mike Boulton

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

This study aimed to evaluate the organelle-specific antioxidant/ pro-oxidant actions of clinically important dietary antioxidants against oxidative stress. An in vitro cellular model was employed to investigate the antioxidant/pro-oxidant effects of various concentrations (1, 10 and 100 μM) of ascorbic acid, α-tocopherol and β-carotene during H2O2-induced oxidative stress. Damage to nuclear and mitochondrial genomes was analyzed by quantitative polymerase chain reaction and oxidation of membrane lipids was measured via colorimetric assays. The key findings were: (i) dietary antioxidants conferred a dose-dependent protective effect (with a pro-oxidant shift at higher concentrations); (ii) the protection conferred to different sub-cellular organelles is highly specific to the dietary antioxidant; (iii) the mtDNA is highly sensitive to oxidative attack compared to nDNA (P <0.05); and (iv) mtDNA protection conferred by dietary antioxidants was required to improve protection against oxidative-induced cell death. This study shows that antioxidant-induced protection of mtDNA is an important target for future oxidative stress therapies.

Original languageEnglish (US)
Pages (from-to)144-152
Number of pages9
JournalRedox Report
Volume11
Issue number4
DOIs
StatePublished - Aug 2006
Externally publishedYes

Fingerprint

Antioxidants
Epithelial Cells
Oxidative stress
Mitochondrial DNA
Reactive Oxygen Species
Oxidative Stress
Organelles
Mitochondrial Genome
Tocopherols
Polymerase chain reaction
Cell death
Carotenoids
Membrane Lipids
Ascorbic Acid
Assays
Cell Death
Genes
Polymerase Chain Reaction
Oxidation

Keywords

  • Β-carotene
  • α-tocopherol
  • Ascorbic acid
  • Lipid peroxidation
  • Mitochondrial DNA damage
  • Nuclear DNA damage

ASJC Scopus subject areas

  • Biochemistry

Cite this

Dietary antioxidants provide differential subcellular protection in epithelial cells. / Jarrett, Stuart G.; Cuenco, Joy; Boulton, Mike.

In: Redox Report, Vol. 11, No. 4, 08.2006, p. 144-152.

Research output: Contribution to journalArticle

Jarrett, Stuart G. ; Cuenco, Joy ; Boulton, Mike. / Dietary antioxidants provide differential subcellular protection in epithelial cells. In: Redox Report. 2006 ; Vol. 11, No. 4. pp. 144-152.
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