Dietary modulation of epidermal growth factor action in cultured pancreatic acinar cells of the rat

P. M. Brannon, A. S. Demarest, J. E. Sabb, Murray Korc

Research output: Contribution to journalArticle

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Abstract

The effects of epidermal growth factor (EGF) and its interaction with dietary adaptation were examined in pancreatic acinar cells isolated from rats fed for 1 wk diets with 67% kcal as fat (HF), 67% kcal as carbohydrate (HC), or unrefined, closed formula diet (UD). Cells were cultured for 48 h in serum-free medium containing 4.2 nM (control), 42 pM, 0.42 nM or 42 nM EGF. EGF at the concentrations tested did not affect cellular protein, DNA, amylase and lipase. In freshly isolated and cultured cells, specific activity of intracellular phenylalanine was affected by diet with the highest specific activity in HF cells, but was not affected by EGF. In freshly isolated cells, EGF increased the rate of phenylalanine incorporation into cellular protein only in HF cells at 42 nM. In cultured cells, EGF biphasically increased phenylalanine incorporation at 42 pM and 42 nM in UD and HC cells, but only increased phenylalanine incorporation in HF cells at 42 nM. These data suggest that diet can alter the response of pancreatic acinar cells to EGF. Dietary alterations of cellular responsiveness to regulatory peptides may participate in pancreatic regulation and dietary adaptation in vivo.

Original languageEnglish (US)
Pages (from-to)1306-1315
Number of pages10
JournalJournal of Nutrition
Volume116
Issue number7
StatePublished - 1986
Externally publishedYes

Fingerprint

acinar cells
Acinar Cells
epidermal growth factor
Epidermal Growth Factor
Phenylalanine
rats
phenylalanine
Cultured Cells
cells
Diet
diet
cultured cells
Formulated Food
Serum-Free Culture Media
Amylases
Lipase
amylases
Proteins
proteins
Fats

ASJC Scopus subject areas

  • Food Science
  • Medicine (miscellaneous)

Cite this

Brannon, P. M., Demarest, A. S., Sabb, J. E., & Korc, M. (1986). Dietary modulation of epidermal growth factor action in cultured pancreatic acinar cells of the rat. Journal of Nutrition, 116(7), 1306-1315.

Dietary modulation of epidermal growth factor action in cultured pancreatic acinar cells of the rat. / Brannon, P. M.; Demarest, A. S.; Sabb, J. E.; Korc, Murray.

In: Journal of Nutrition, Vol. 116, No. 7, 1986, p. 1306-1315.

Research output: Contribution to journalArticle

Brannon, PM, Demarest, AS, Sabb, JE & Korc, M 1986, 'Dietary modulation of epidermal growth factor action in cultured pancreatic acinar cells of the rat', Journal of Nutrition, vol. 116, no. 7, pp. 1306-1315.
Brannon, P. M. ; Demarest, A. S. ; Sabb, J. E. ; Korc, Murray. / Dietary modulation of epidermal growth factor action in cultured pancreatic acinar cells of the rat. In: Journal of Nutrition. 1986 ; Vol. 116, No. 7. pp. 1306-1315.
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