Dietary protein restriction inhibits tumor growth in human xenograft models of prostate and breast cancer

Luigi Fontana, Remi M. Adelaiye, Antonella L. Rastelli, Kiersten Marie Miles, Eric Ciamporcero, Valter D. Longo, Holly Nguyen, Robert Vessella, Roberto Pili

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Purpose: Data from epidemiological and experimental studies suggest that dietary protein intake may play a role in inhibiting prostate and breast cancer by modulating the IGF/AKT/mTOR pathway. In this study we investigated the effects of diets with different protein content or quality on prostate and breast cancer. Experimental Design: To test our hypothesis we assessed the inhibitory effect of protein diet restriction on prostate and breast cancer growth, serum PSA and IGF-1 concentrations, mTOR activity and epigenetic markers, by using human xenograft cancer models. Results: Our results showed a 70% inhibition of tumor growth in the castrate-resistant LuCaP23.1 prostate cancer model and a 56% inhibition in the WHIM16 breast cancer model fed with a 7% protein diet when compared to an isocaloric 21% protein diet. Inhibition of tumor growth correlated, in the LuCaP23.1 model, with decreased serum PSA and IGF-1 levels, down-regulation of mTORC1 activity, decreased cell proliferation as indicated by Ki67 staining, and reduction in epigenetic markers of prostate cancer progression, including the histone methyltransferase EZH2 and the associated histone mark H3K27me3. In addition, we observed that modifications of dietary protein quality, independently of protein quantity, decreased tumor growth. A diet containing 20% plant protein inhibited tumor weight by 37% as compared to a 20% animal dairy protein diet. Conclusions: Our findings suggest that a reduction in dietary protein intake is highly effective in inhibiting tumor growth in human xenograft prostate and breast cancer models, possibly through the inhibition of the IGF/AKT/mTOR pathway and epigenetic modifications.

Original languageEnglish (US)
Pages (from-to)2451-2461
Number of pages11
JournalOncotarget
Volume4
Issue number12
StatePublished - Dec 2013
Externally publishedYes

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Dietary Proteins
Heterografts
Prostatic Neoplasms
Breast Neoplasms
Diet
Growth
Epigenomics
Neoplasms
Proteins
Insulin-Like Growth Factor I
Histone Code
Plant Proteins
Tumor Burden
Serum
Epidemiologic Studies
Research Design
Down-Regulation
Cell Proliferation
Staining and Labeling

Keywords

  • mTOR
  • Prostate and breast cancer
  • Protein restriction

ASJC Scopus subject areas

  • Oncology

Cite this

Fontana, L., Adelaiye, R. M., Rastelli, A. L., Miles, K. M., Ciamporcero, E., Longo, V. D., ... Pili, R. (2013). Dietary protein restriction inhibits tumor growth in human xenograft models of prostate and breast cancer. Oncotarget, 4(12), 2451-2461.

Dietary protein restriction inhibits tumor growth in human xenograft models of prostate and breast cancer. / Fontana, Luigi; Adelaiye, Remi M.; Rastelli, Antonella L.; Miles, Kiersten Marie; Ciamporcero, Eric; Longo, Valter D.; Nguyen, Holly; Vessella, Robert; Pili, Roberto.

In: Oncotarget, Vol. 4, No. 12, 12.2013, p. 2451-2461.

Research output: Contribution to journalArticle

Fontana, L, Adelaiye, RM, Rastelli, AL, Miles, KM, Ciamporcero, E, Longo, VD, Nguyen, H, Vessella, R & Pili, R 2013, 'Dietary protein restriction inhibits tumor growth in human xenograft models of prostate and breast cancer', Oncotarget, vol. 4, no. 12, pp. 2451-2461.
Fontana L, Adelaiye RM, Rastelli AL, Miles KM, Ciamporcero E, Longo VD et al. Dietary protein restriction inhibits tumor growth in human xenograft models of prostate and breast cancer. Oncotarget. 2013 Dec;4(12):2451-2461.
Fontana, Luigi ; Adelaiye, Remi M. ; Rastelli, Antonella L. ; Miles, Kiersten Marie ; Ciamporcero, Eric ; Longo, Valter D. ; Nguyen, Holly ; Vessella, Robert ; Pili, Roberto. / Dietary protein restriction inhibits tumor growth in human xenograft models of prostate and breast cancer. In: Oncotarget. 2013 ; Vol. 4, No. 12. pp. 2451-2461.
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