Dietary salt exacerbates isoproterenol-induced cardiomyopathy in rats

Alex P. Carll, Najwa Haykal-Coates, Darrell W. Winsett, Mehdi S. Hazari, Abraham Nyska, Judy H. Richards, Monte Willis, Daniel L. Costa, Aimen K. Farraj

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Spontaneously hypertensive heart failure rats (SHHFs) take longer to develop compensated heart failure (HF) and congestive decompensation than common surgical models of HF. Isoproterenol (ISO) infusion can accelerate cardiomyopathy in young SHHFs, while dietary salt loading in hypertensive rats induces cardiac fibrosis, hypertrophy, and-in a minority-congestive HF. By combining ISO with dietary salt loading in young SHHFs, the authors sought a nonsurgical model that is more time- and resource-efficient than any of these factors alone. The authors hypothesized that salt loading would enhance ISO-accelerated cardiomyopathy, promoting fibrosis, hypertrophy, and biochemical characteristics of HF. SHHFs (lean male, 90d) were infused for 4 wk with ISO (2.5 mg/kg/day) or saline. After 2 wk of infusion, a 6-wk high-salt diet (4%, 6%, or 8% NaCl) was initiated. Eight percent salt increased heart weight, HF markers (plasma B-type natriuretic peptide, IL-6), lung lymphocytes, and indicators of lung injury and edema (albumin and protein) relative to control diet, while increasing urine pro-atrial natriuretic peptide relative to ISO-only. High salt also exacerbated ISO-cardiomyopathy and fibrosis. Thus, combining ISO infusion with dietary salt loading in SHHFs holds promise for a new rat HF model that may help researchers to elucidate HF mechanisms and unearth effective treatments.

Original languageEnglish (US)
Pages (from-to)925-937
Number of pages13
JournalToxicologic Pathology
Volume39
Issue number6
DOIs
StatePublished - Oct 1 2011
Externally publishedYes

Fingerprint

Cardiomyopathies
Isoproterenol
Rats
Heart Failure
Salts
Fibrosis
Nutrition
Diet
Anatomic Models
Brain Natriuretic Peptide
Cardiomegaly
Lymphocytes
Lung Injury
Atrial Natriuretic Factor
Hypertrophy
Albumins
Interleukin-6
Edema
Research Personnel
Urine

Keywords

  • cardiomyopathy; salt; isoproterenol; heart failure; spontaneously hypertensive; rats; fibrosis

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Molecular Biology
  • Toxicology
  • Cell Biology

Cite this

Carll, A. P., Haykal-Coates, N., Winsett, D. W., Hazari, M. S., Nyska, A., Richards, J. H., ... Farraj, A. K. (2011). Dietary salt exacerbates isoproterenol-induced cardiomyopathy in rats. Toxicologic Pathology, 39(6), 925-937. https://doi.org/10.1177/0192623311416373

Dietary salt exacerbates isoproterenol-induced cardiomyopathy in rats. / Carll, Alex P.; Haykal-Coates, Najwa; Winsett, Darrell W.; Hazari, Mehdi S.; Nyska, Abraham; Richards, Judy H.; Willis, Monte; Costa, Daniel L.; Farraj, Aimen K.

In: Toxicologic Pathology, Vol. 39, No. 6, 01.10.2011, p. 925-937.

Research output: Contribution to journalArticle

Carll, AP, Haykal-Coates, N, Winsett, DW, Hazari, MS, Nyska, A, Richards, JH, Willis, M, Costa, DL & Farraj, AK 2011, 'Dietary salt exacerbates isoproterenol-induced cardiomyopathy in rats', Toxicologic Pathology, vol. 39, no. 6, pp. 925-937. https://doi.org/10.1177/0192623311416373
Carll AP, Haykal-Coates N, Winsett DW, Hazari MS, Nyska A, Richards JH et al. Dietary salt exacerbates isoproterenol-induced cardiomyopathy in rats. Toxicologic Pathology. 2011 Oct 1;39(6):925-937. https://doi.org/10.1177/0192623311416373
Carll, Alex P. ; Haykal-Coates, Najwa ; Winsett, Darrell W. ; Hazari, Mehdi S. ; Nyska, Abraham ; Richards, Judy H. ; Willis, Monte ; Costa, Daniel L. ; Farraj, Aimen K. / Dietary salt exacerbates isoproterenol-induced cardiomyopathy in rats. In: Toxicologic Pathology. 2011 ; Vol. 39, No. 6. pp. 925-937.
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