Differences among mononuclear cell subpopulations in hiv seropositive or seronegative homosexual and heterosexual men as determined by four-color flow cytometry

Carol T. Schnizlein-Bick, Marcie R. Magier, Robert B. Jones, Kenneth H. Fife, Barry P. Katz, Edwin B. Walker

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

Four-color cell surface immunofluorescence and flow cytometry analysis was used to quantitate mononuclear cell subpopulations from HIV seropositive (HIV<sup>+</sup>) and seronegative (HIV<sup>−</sup>) homosexual men and heterosexual men. HIV<sup>+</sup> men were divided into two groups based on peripheral blood CD4/mm<sup>3</sup> of >500 or <500. CD4<sup>+</sup> cells that were simultaneously CD45R<sup>−</sup>, CDw29<sup>−</sup>, and 13<sup>−</sup> were significantly less in HIV<sup>+</sup> men with <500 CD4/mm<sup>3</sup> (17%) compared to heterosexual men (34%). This lower percentage of “CD4 only” cells in HIV<sup>+</sup> males with <500 CD4/mm<sup>3</sup> correlated with a significantly higher percentage of CD4<sup>+</sup> cells that were CD45R<sup>+</sup>, CDw29<sup>+</sup>, and 13<sup>+</sup> in these individuals. CD8<sup>+</sup> cells that were CD45R<sup>+</sup>, 13<sup>+</sup>, but CD38<sup>−</sup>, were significantly less in HIV<sup>+</sup> men with <500 CD4 as compared to HIV<sup>−</sup>homosexual men. In contrast, a second CD8<sup>+</sup> subpopulation that was CD45R<sup>−</sup>, CD38<sup>+</sup>, and either 13<sup>+</sup> or 13<sup>−</sup> was significantly greater in <500 HIV<sup>+</sup> men as compared to both HIV<sup>−</sup> homosexual men and heterosexual men. A significant difference in this subpopulation was observed between the <500 and >500 HIV<sup>+</sup> groups and correlated with seropositivity for viral p24 antigen. Interestingly, CD8<sup>+</sup> cells that were CD45R<sup>+</sup>, as well as CD38<sup>+</sup>, and either 13<sup>+</sup> or 13 ˜˜were significantly greater in the <500 HIV<sup>+</sup> group compared to the >500 HIV<sup>+</sup> group, and did not correlate with p24 seropositivity. The percentage of monocyte/macrophages that were CD4<sup>−</sup> or expressed dim CD4 immunofluorescence, but were 13<sup>+</sup>, was significantly greater in HIV<sup>+</sup> men (43%) compared to HIV<sup>−</sup> homosexual men (27%). In summary, we have identified previously undescribed mononuclear cell subpopulations that were altered with HIV infection and, in some cases, correlated with the stage of disease.

Original languageEnglish (US)
Pages (from-to)747-756
Number of pages10
JournalJournal of Acquired Immune Deficiency Syndromes
Volume3
Issue number8
StatePublished - Aug 1990

Keywords

  • Flow cytometry
  • Immunological status
  • Lymphocytes
  • Monocytes
  • Surface antigens

ASJC Scopus subject areas

  • Infectious Diseases
  • Pharmacology (medical)
  • Immunology and Allergy
  • Virology

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