Differences in the lower airway microbiota of infants with and without cystic fibrosis

Katherine B. Frayman, Kristine M. Wylie, David S. Armstrong, Rosemary Carzino, Stephanie Davis, Thomas W. Ferkol, Keith Grimwood, Gregory A. Storch, Sarath C. Ranganathan

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Background: Cystic fibrosis (CF) lung disease commences in infancy, and understanding the role of the microbiota in disease pathogenesis is critical. This study examined and compared the lower airway microbiota of infants with and without CF and its relationship to airway inflammation in the first months of life. Methods: Infants newly-diagnosed with CF were recruited into a single-centre study in Melbourne, Australia from 1992 to 2001. Bronchoalveolar lavage was performed at study entry. Healthy infants undergoing bronchoscopy to investigate chronic stridor acted as controls. Quantitative microbiological culture was performed and inflammatory markers were measured contemporaneously. 16S ribosomal RNA gene analysis was performed on stored samples. Results: Thirteen bronchoalveolar samples from infants with CF and nine from control infants, collected at median ages of 1.8-months (25th–75th percentile 1.5 to 3.1-months) and 5-months (25th–75th percentile 2.9 to 8.2-months) respectively, provided 16S rRNA gene data. Bacterial biomass was positively associated with inflammation. Alpha diversity was reduced in infants with CF and between-group compositional differences were apparent. These differences were driven by increased Staphylococcus and decreased Fusobacterium and were most apparent in symptomatic infants with CF. Conclusion: In CF lung disease, differences in lower airway microbial community composition and structure are established by age 6-months.

Original languageEnglish (US)
JournalJournal of Cystic Fibrosis
DOIs
StateAccepted/In press - Jan 1 2018
Externally publishedYes

Fingerprint

Microbiota
Cystic Fibrosis
rRNA Genes
Lung Diseases
Fusobacterium
16S Ribosomal RNA
Inflammation
Respiratory Sounds
Bronchoscopy
Bronchoalveolar Lavage
Staphylococcus
Biomass

Keywords

  • 16S ribosomal RNA
  • Bronchoalveolar lavage fluid
  • Cystic fibrosis
  • Infant
  • Inflammation
  • Microbiota

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Pulmonary and Respiratory Medicine

Cite this

Frayman, K. B., Wylie, K. M., Armstrong, D. S., Carzino, R., Davis, S., Ferkol, T. W., ... Ranganathan, S. C. (Accepted/In press). Differences in the lower airway microbiota of infants with and without cystic fibrosis. Journal of Cystic Fibrosis. https://doi.org/10.1016/j.jcf.2018.12.003

Differences in the lower airway microbiota of infants with and without cystic fibrosis. / Frayman, Katherine B.; Wylie, Kristine M.; Armstrong, David S.; Carzino, Rosemary; Davis, Stephanie; Ferkol, Thomas W.; Grimwood, Keith; Storch, Gregory A.; Ranganathan, Sarath C.

In: Journal of Cystic Fibrosis, 01.01.2018.

Research output: Contribution to journalArticle

Frayman, KB, Wylie, KM, Armstrong, DS, Carzino, R, Davis, S, Ferkol, TW, Grimwood, K, Storch, GA & Ranganathan, SC 2018, 'Differences in the lower airway microbiota of infants with and without cystic fibrosis', Journal of Cystic Fibrosis. https://doi.org/10.1016/j.jcf.2018.12.003
Frayman, Katherine B. ; Wylie, Kristine M. ; Armstrong, David S. ; Carzino, Rosemary ; Davis, Stephanie ; Ferkol, Thomas W. ; Grimwood, Keith ; Storch, Gregory A. ; Ranganathan, Sarath C. / Differences in the lower airway microbiota of infants with and without cystic fibrosis. In: Journal of Cystic Fibrosis. 2018.
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