Different blocking agents cause variation in the immunologic detection of proteins transferred to nitrocellulose membranes

Stanley M. Spinola, Janne G. Cannon

Research output: Contribution to journalArticle

48 Scopus citations

Abstract

We compared bovine serum albumin, commercial non-fat dry milk, and Tween 20 as blocking agents for immunologic probing of bacterial proteins transferred to nitrocellulose sheets. There were quantitative and qualitative differences in antigens detected that depended on which blocking agents were used. We suggest that several methods for blocking and washing nitrocellulose should be compared when Western blotting is used to detect immunologically reactive proteins.

Original languageEnglish (US)
Pages (from-to)161-165
Number of pages5
JournalJournal of Immunological Methods
Volume81
Issue number1
DOIs
StatePublished - Jul 16 1985
Externally publishedYes

Keywords

  • Western blotting
  • blocking agents

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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