Different effect of proteasome inhibition on vesicular stomatitis virus and poliovirus replication

Nickolay Neznanov, Eugenia M. Dragunsky, Konstantin M. Chumakov, Lubov Neznanova, Ronald Wek, Andrei V. Gudkov, Amiya K. Banerjee

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Proteasome activity is an important part of viral replication. In this study, we examined the effect of proteasome inhibitors on the replication of vesicular stomatitis virus (VSV) and poliovirus. We found that the proteasome inhibitors significantly suppressed VSV protein synthesis, virus accumulation, and protected infected cells from toxic effect of VSV replication. In contrast, poliovirus replication was delayed, but not diminished in the presence of the proteasome inhibitors MG132 and Bortezomib. We also found that inhibition of proteasomes stimulated stress-related processes, such as accumulation of chaperone hsp70, phosphorylation of eIF2α, and overall inhibition of translation. VSV replication was sensitive to this stress with significant decline in replication process. Poliovirus growth was less sensitive with only delay in replication. Inhibition of proteasome activity suppressed cellular and VSV pretein synthesis, but did not reduce poliovirus protein synthesis. Protein kinase GCN2 supported the ability of proteasome inhibitors to attenuate general translation and to suppress VSV replication. We propose that different mechanisms of translational initiation by VSV and poliovirus determine their sensitivity to stress induced by the inhibition of proteasomes. To our knowledge, this is the first study that connects the effect of stress induced by proteasome inhibition with the efficiency of viral infection.

Original languageEnglish
Article numbere1887
JournalPLoS One
Volume3
Issue number4
DOIs
StatePublished - Apr 2 2008

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Vesiculovirus
Enterovirus C
Vesicular Stomatitis
Poliovirus
proteasome endopeptidase complex
Proteasome Endopeptidase Complex
Virus Replication
Viruses
Proteasome Inhibitors
virus replication
translation (genetics)
protein synthesis
Poisons
Phosphorylation
Virus Diseases
Protein Kinases
Proteins
protein kinases
phosphorylation
Growth

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Neznanov, N., Dragunsky, E. M., Chumakov, K. M., Neznanova, L., Wek, R., Gudkov, A. V., & Banerjee, A. K. (2008). Different effect of proteasome inhibition on vesicular stomatitis virus and poliovirus replication. PLoS One, 3(4), [e1887]. https://doi.org/10.1371/journal.pone.0001887

Different effect of proteasome inhibition on vesicular stomatitis virus and poliovirus replication. / Neznanov, Nickolay; Dragunsky, Eugenia M.; Chumakov, Konstantin M.; Neznanova, Lubov; Wek, Ronald; Gudkov, Andrei V.; Banerjee, Amiya K.

In: PLoS One, Vol. 3, No. 4, e1887, 02.04.2008.

Research output: Contribution to journalArticle

Neznanov, N, Dragunsky, EM, Chumakov, KM, Neznanova, L, Wek, R, Gudkov, AV & Banerjee, AK 2008, 'Different effect of proteasome inhibition on vesicular stomatitis virus and poliovirus replication', PLoS One, vol. 3, no. 4, e1887. https://doi.org/10.1371/journal.pone.0001887
Neznanov, Nickolay ; Dragunsky, Eugenia M. ; Chumakov, Konstantin M. ; Neznanova, Lubov ; Wek, Ronald ; Gudkov, Andrei V. ; Banerjee, Amiya K. / Different effect of proteasome inhibition on vesicular stomatitis virus and poliovirus replication. In: PLoS One. 2008 ; Vol. 3, No. 4.
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