Differential β-adrenoceptor expression induced by nerve growth factor infusion into the canine right and left stellate ganglia

Shengmei Zhou, Offir Paz, Ji Min Cao, Kamlesh Asotra, Ning Ning Chai, Charles Wang, Lan Chen, Michael C. Fishbein, Behrooz Sharifi, Peng-Sheng Chen

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Background: Nerve growth factor (NGF) infusion into the right stellate ganglion (RSG) is antiarrhythmic, while NGF infusion into the left stellate ganglion (LSG) is proarrhythmic in dogs with myocardial infarction (MI) and complete atrioventricular block (CAVB). This functional asymmetry suggests differential neural remodeling. Objectives: To test the hypothesis that NGF infusion into the RSG and the LSG can lead to differential β-adrenoceptor (β-AR) expression in dogs with MI and CAVB. Methods and results: We performed immunostaining to quantify β1-AR and β3-AR immunoreactivity in six dogs with MI and CAVB, nine dogs with MI, CAVB, and NGF infusion to the LSG, six dogs with MI, CAVB, and NGF infusion to the RSG, and six normal dogs. There was significantly increased β3-AR immunoreactivity in dogs with NGF infusion into the LSG and significantly decreased β3-AR immunoreactivity in dogs with NGF infusion into the RSG compared with controls and with the MI and CAVB group. There were no significant differences in β1-AR immunoreactivity among these four groups. To determine protein and mRNA expression of β-ARs, we created MI and CAVB and infused NGF into the LSG in six additional dogs. The noninfarcted left ventricle free wall was harvested 1 week later. The protein level and receptor density of β3-AR (but not β1- or β2-AR) significantly increased in these six dogs compared with normal controls. Conclusions: We conclude that NGF infusion into the RSG and the LSG in dogs with MI and CAVB induced differential β3-AR expression in the left ventricular myocardium.

Original languageEnglish (US)
Pages (from-to)1347-1355
Number of pages9
JournalHeart Rhythm
Volume2
Issue number12
DOIs
StatePublished - Dec 2005
Externally publishedYes

Fingerprint

Stellate Ganglion
Nerve Growth Factor
Adrenergic Receptors
Canidae
Atrioventricular Block
Dogs
Myocardial Infarction
Heart Ventricles
Myocardium
Proteins

Keywords

  • Arrhythmia
  • Death, sudden
  • Nervous system, sympathetic
  • Receptors, adrenergic, beta

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Differential β-adrenoceptor expression induced by nerve growth factor infusion into the canine right and left stellate ganglia. / Zhou, Shengmei; Paz, Offir; Cao, Ji Min; Asotra, Kamlesh; Chai, Ning Ning; Wang, Charles; Chen, Lan; Fishbein, Michael C.; Sharifi, Behrooz; Chen, Peng-Sheng.

In: Heart Rhythm, Vol. 2, No. 12, 12.2005, p. 1347-1355.

Research output: Contribution to journalArticle

Zhou, Shengmei ; Paz, Offir ; Cao, Ji Min ; Asotra, Kamlesh ; Chai, Ning Ning ; Wang, Charles ; Chen, Lan ; Fishbein, Michael C. ; Sharifi, Behrooz ; Chen, Peng-Sheng. / Differential β-adrenoceptor expression induced by nerve growth factor infusion into the canine right and left stellate ganglia. In: Heart Rhythm. 2005 ; Vol. 2, No. 12. pp. 1347-1355.
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abstract = "Background: Nerve growth factor (NGF) infusion into the right stellate ganglion (RSG) is antiarrhythmic, while NGF infusion into the left stellate ganglion (LSG) is proarrhythmic in dogs with myocardial infarction (MI) and complete atrioventricular block (CAVB). This functional asymmetry suggests differential neural remodeling. Objectives: To test the hypothesis that NGF infusion into the RSG and the LSG can lead to differential β-adrenoceptor (β-AR) expression in dogs with MI and CAVB. Methods and results: We performed immunostaining to quantify β1-AR and β3-AR immunoreactivity in six dogs with MI and CAVB, nine dogs with MI, CAVB, and NGF infusion to the LSG, six dogs with MI, CAVB, and NGF infusion to the RSG, and six normal dogs. There was significantly increased β3-AR immunoreactivity in dogs with NGF infusion into the LSG and significantly decreased β3-AR immunoreactivity in dogs with NGF infusion into the RSG compared with controls and with the MI and CAVB group. There were no significant differences in β1-AR immunoreactivity among these four groups. To determine protein and mRNA expression of β-ARs, we created MI and CAVB and infused NGF into the LSG in six additional dogs. The noninfarcted left ventricle free wall was harvested 1 week later. The protein level and receptor density of β3-AR (but not β1- or β2-AR) significantly increased in these six dogs compared with normal controls. Conclusions: We conclude that NGF infusion into the RSG and the LSG in dogs with MI and CAVB induced differential β3-AR expression in the left ventricular myocardium.",
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author = "Shengmei Zhou and Offir Paz and Cao, {Ji Min} and Kamlesh Asotra and Chai, {Ning Ning} and Charles Wang and Lan Chen and Fishbein, {Michael C.} and Behrooz Sharifi and Peng-Sheng Chen",
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T1 - Differential β-adrenoceptor expression induced by nerve growth factor infusion into the canine right and left stellate ganglia

AU - Zhou, Shengmei

AU - Paz, Offir

AU - Cao, Ji Min

AU - Asotra, Kamlesh

AU - Chai, Ning Ning

AU - Wang, Charles

AU - Chen, Lan

AU - Fishbein, Michael C.

AU - Sharifi, Behrooz

AU - Chen, Peng-Sheng

PY - 2005/12

Y1 - 2005/12

N2 - Background: Nerve growth factor (NGF) infusion into the right stellate ganglion (RSG) is antiarrhythmic, while NGF infusion into the left stellate ganglion (LSG) is proarrhythmic in dogs with myocardial infarction (MI) and complete atrioventricular block (CAVB). This functional asymmetry suggests differential neural remodeling. Objectives: To test the hypothesis that NGF infusion into the RSG and the LSG can lead to differential β-adrenoceptor (β-AR) expression in dogs with MI and CAVB. Methods and results: We performed immunostaining to quantify β1-AR and β3-AR immunoreactivity in six dogs with MI and CAVB, nine dogs with MI, CAVB, and NGF infusion to the LSG, six dogs with MI, CAVB, and NGF infusion to the RSG, and six normal dogs. There was significantly increased β3-AR immunoreactivity in dogs with NGF infusion into the LSG and significantly decreased β3-AR immunoreactivity in dogs with NGF infusion into the RSG compared with controls and with the MI and CAVB group. There were no significant differences in β1-AR immunoreactivity among these four groups. To determine protein and mRNA expression of β-ARs, we created MI and CAVB and infused NGF into the LSG in six additional dogs. The noninfarcted left ventricle free wall was harvested 1 week later. The protein level and receptor density of β3-AR (but not β1- or β2-AR) significantly increased in these six dogs compared with normal controls. Conclusions: We conclude that NGF infusion into the RSG and the LSG in dogs with MI and CAVB induced differential β3-AR expression in the left ventricular myocardium.

AB - Background: Nerve growth factor (NGF) infusion into the right stellate ganglion (RSG) is antiarrhythmic, while NGF infusion into the left stellate ganglion (LSG) is proarrhythmic in dogs with myocardial infarction (MI) and complete atrioventricular block (CAVB). This functional asymmetry suggests differential neural remodeling. Objectives: To test the hypothesis that NGF infusion into the RSG and the LSG can lead to differential β-adrenoceptor (β-AR) expression in dogs with MI and CAVB. Methods and results: We performed immunostaining to quantify β1-AR and β3-AR immunoreactivity in six dogs with MI and CAVB, nine dogs with MI, CAVB, and NGF infusion to the LSG, six dogs with MI, CAVB, and NGF infusion to the RSG, and six normal dogs. There was significantly increased β3-AR immunoreactivity in dogs with NGF infusion into the LSG and significantly decreased β3-AR immunoreactivity in dogs with NGF infusion into the RSG compared with controls and with the MI and CAVB group. There were no significant differences in β1-AR immunoreactivity among these four groups. To determine protein and mRNA expression of β-ARs, we created MI and CAVB and infused NGF into the LSG in six additional dogs. The noninfarcted left ventricle free wall was harvested 1 week later. The protein level and receptor density of β3-AR (but not β1- or β2-AR) significantly increased in these six dogs compared with normal controls. Conclusions: We conclude that NGF infusion into the RSG and the LSG in dogs with MI and CAVB induced differential β3-AR expression in the left ventricular myocardium.

KW - Arrhythmia

KW - Death, sudden

KW - Nervous system, sympathetic

KW - Receptors, adrenergic, beta

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