Differential and specific labeling of epithelial and vascular endothelial cells of the rat lung by Lycopersicon esculentum and Griffonia simplicifolia I lectins

P. W. Bankston, G. A. Porter, A. J. Milici, G. E. Palade

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

In the rat lung, we found that the Lycopersicon esculentum (LEA) lectin specifically binds to the epithelium of bronchioles and alveoli whereas Griffonia simplicifolia I (GS-I) binds to the endothelium of alveolar capillaries. The differential binding affinity of these lectins was examined on semithin (~ 0.5 μ) and thin (< 0.1 μm) frozen sections of rat lung lavaged to remove alveolar macrophages. On semithin frozen sections, LEA bound to epithelial cells lining bronchioles and the alveoli (type I, but not type II epithelial cells). On thin frozen sections, biotinylated Lycopersicon esculentum (bLEA)-streptavidin-gold conjugates were confined primarily to the luminal plasmalemma of type I cells. bGS-I-streptavidin-Texas Red was detected on the endothelial cells of alveolar capillaries and postcapillary venules but not on those of larger venules, veins or arterioles. By electron microscopy, GS-I-streptavidin-gold complexes were localized primarily to the luminal plasmalemma of thick and thin regions of the capillary endothelium. Neither lectin labeled type II alveolar cells, but both lectins labeled macrophages in the interstitia and in incompletely lavaged alveoli.

Original languageEnglish (US)
Pages (from-to)187-195
Number of pages9
JournalEuropean Journal of Cell Biology
Volume54
Issue number2
StatePublished - May 16 1991

Fingerprint

Streptavidin
Frozen Sections
Lycopersicon esculentum
Griffonia
Lectins
Bronchioles
Venules
Endothelial Cells
Vascular Endothelium
Gold
Lung
Epithelial Cells
Alveolar Epithelial Cells
Alveolar Macrophages
Arterioles
Veins
Electron Microscopy
Epithelium
Macrophages
Griffonia simplicifolia lectins

Keywords

  • lectin
  • lung
  • microvascular endothelium
  • type I epithelial cells

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Differential and specific labeling of epithelial and vascular endothelial cells of the rat lung by Lycopersicon esculentum and Griffonia simplicifolia I lectins. / Bankston, P. W.; Porter, G. A.; Milici, A. J.; Palade, G. E.

In: European Journal of Cell Biology, Vol. 54, No. 2, 16.05.1991, p. 187-195.

Research output: Contribution to journalArticle

@article{3294b9d024da4a4cb3900d856491615a,
title = "Differential and specific labeling of epithelial and vascular endothelial cells of the rat lung by Lycopersicon esculentum and Griffonia simplicifolia I lectins",
abstract = "In the rat lung, we found that the Lycopersicon esculentum (LEA) lectin specifically binds to the epithelium of bronchioles and alveoli whereas Griffonia simplicifolia I (GS-I) binds to the endothelium of alveolar capillaries. The differential binding affinity of these lectins was examined on semithin (~ 0.5 μ) and thin (< 0.1 μm) frozen sections of rat lung lavaged to remove alveolar macrophages. On semithin frozen sections, LEA bound to epithelial cells lining bronchioles and the alveoli (type I, but not type II epithelial cells). On thin frozen sections, biotinylated Lycopersicon esculentum (bLEA)-streptavidin-gold conjugates were confined primarily to the luminal plasmalemma of type I cells. bGS-I-streptavidin-Texas Red was detected on the endothelial cells of alveolar capillaries and postcapillary venules but not on those of larger venules, veins or arterioles. By electron microscopy, GS-I-streptavidin-gold complexes were localized primarily to the luminal plasmalemma of thick and thin regions of the capillary endothelium. Neither lectin labeled type II alveolar cells, but both lectins labeled macrophages in the interstitia and in incompletely lavaged alveoli.",
keywords = "lectin, lung, microvascular endothelium, type I epithelial cells",
author = "Bankston, {P. W.} and Porter, {G. A.} and Milici, {A. J.} and Palade, {G. E.}",
year = "1991",
month = "5",
day = "16",
language = "English (US)",
volume = "54",
pages = "187--195",
journal = "European Journal of Cell Biology",
issn = "0171-9335",
publisher = "Urban und Fischer Verlag GmbH und Co. KG",
number = "2",

}

TY - JOUR

T1 - Differential and specific labeling of epithelial and vascular endothelial cells of the rat lung by Lycopersicon esculentum and Griffonia simplicifolia I lectins

AU - Bankston, P. W.

AU - Porter, G. A.

AU - Milici, A. J.

AU - Palade, G. E.

PY - 1991/5/16

Y1 - 1991/5/16

N2 - In the rat lung, we found that the Lycopersicon esculentum (LEA) lectin specifically binds to the epithelium of bronchioles and alveoli whereas Griffonia simplicifolia I (GS-I) binds to the endothelium of alveolar capillaries. The differential binding affinity of these lectins was examined on semithin (~ 0.5 μ) and thin (< 0.1 μm) frozen sections of rat lung lavaged to remove alveolar macrophages. On semithin frozen sections, LEA bound to epithelial cells lining bronchioles and the alveoli (type I, but not type II epithelial cells). On thin frozen sections, biotinylated Lycopersicon esculentum (bLEA)-streptavidin-gold conjugates were confined primarily to the luminal plasmalemma of type I cells. bGS-I-streptavidin-Texas Red was detected on the endothelial cells of alveolar capillaries and postcapillary venules but not on those of larger venules, veins or arterioles. By electron microscopy, GS-I-streptavidin-gold complexes were localized primarily to the luminal plasmalemma of thick and thin regions of the capillary endothelium. Neither lectin labeled type II alveolar cells, but both lectins labeled macrophages in the interstitia and in incompletely lavaged alveoli.

AB - In the rat lung, we found that the Lycopersicon esculentum (LEA) lectin specifically binds to the epithelium of bronchioles and alveoli whereas Griffonia simplicifolia I (GS-I) binds to the endothelium of alveolar capillaries. The differential binding affinity of these lectins was examined on semithin (~ 0.5 μ) and thin (< 0.1 μm) frozen sections of rat lung lavaged to remove alveolar macrophages. On semithin frozen sections, LEA bound to epithelial cells lining bronchioles and the alveoli (type I, but not type II epithelial cells). On thin frozen sections, biotinylated Lycopersicon esculentum (bLEA)-streptavidin-gold conjugates were confined primarily to the luminal plasmalemma of type I cells. bGS-I-streptavidin-Texas Red was detected on the endothelial cells of alveolar capillaries and postcapillary venules but not on those of larger venules, veins or arterioles. By electron microscopy, GS-I-streptavidin-gold complexes were localized primarily to the luminal plasmalemma of thick and thin regions of the capillary endothelium. Neither lectin labeled type II alveolar cells, but both lectins labeled macrophages in the interstitia and in incompletely lavaged alveoli.

KW - lectin

KW - lung

KW - microvascular endothelium

KW - type I epithelial cells

UR - http://www.scopus.com/inward/record.url?scp=0025754060&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025754060&partnerID=8YFLogxK

M3 - Article

C2 - 1879433

AN - SCOPUS:0025754060

VL - 54

SP - 187

EP - 195

JO - European Journal of Cell Biology

JF - European Journal of Cell Biology

SN - 0171-9335

IS - 2

ER -