Differential down-regulation of CD95 or CD95L in chronically HIV-infected cells of monocytic or lymphocytic origin

Cellular studies and molecular analysis by quantitative competitive RT-PCR

Marcello Pinti, Jessica Pedrazzi, Francesca Benatti, Veronica Sorrentino, Cira Nuzzo, Valeria Cavazzuti, Priscilla Biswas, Daniela Petrusca, Cristina Mussini, Bruno De Rienzo, Andrea Cossarizza

Research output: Contribution to journalArticle

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Abstract

We analysed the expression of CD95/CD95L in two widely used models for studying the cellular effects of chronic infection with human immunodeficiency virus type 1 (HIV-1), i.e. ACH-2 cells, derived from the lymphocytic cell line A301, and U1, derived from monocytic U937 cells. A301 and ACH-2 mounted the same amount of plasma membrane CD95, while U1 had a consistent decrease in CD95 expression. Using different antibodies, we failed to detect the plasma membrane form of its ligand, CD95L, but we could see the intracellular presence of that molecule in A301 cells and, to a lesser extent, in ACH-2 cells, but not in U937 or U1 cells. To confirm the cytofluorimetric data and quantify the expression of CD95L at the RNA level, we developed a quantitative competitive RT-PCR assay. The HUT78 cell line had about 50 000 copies mRNA/1000 cells, three times more after induction with a phorbol ester and ionomycin. ACH-2 expressed about 400- (basal) or 10- (induced) fold less CD95L mRNA than the parental cell line A301; U937 and U1 were below the limit of detection. In cells of lymphoid origin (ACH-2) chronic HIV infection inhibits the expression of CD95L, the phenomenon occurring at the transcriptional level. In cells of monocytic origin (U1) the infection decreases the plasma membrane expression of CD95. This suggests that HIV could trigger different anti-apoptotic strategies which likely depend upon the cell line which is infected. In monocytic cells which act as a viral reservoir, the expression of the molecule whose binding triggers apoptosis decreases, while in lymphoid cells, capable of exerting cytotoxicity, the expression of a molecule which induces apoptosis is reduced. Copyright (C) 1999 Federation of European Biochemical Societies.

Original languageEnglish (US)
Pages (from-to)209-214
Number of pages6
JournalFEBS Letters
Volume458
Issue number2
DOIs
StatePublished - Sep 17 1999
Externally publishedYes

Fingerprint

Fas Ligand Protein
Norgestrienone
Down-Regulation
HIV
Cell membranes
Polymerase Chain Reaction
Cells
Cell Line
Molecules
Cell Membrane
Apoptosis
Messenger RNA
Ionomycin
Phorbol Esters
Cytotoxicity
Lymphocytes
Viruses
U937 Cells
Assays
Infection

Keywords

  • AIDS
  • Apoptosis
  • CD95L
  • FasL
  • HIV
  • Quantitative competitive RT-PCR

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Differential down-regulation of CD95 or CD95L in chronically HIV-infected cells of monocytic or lymphocytic origin : Cellular studies and molecular analysis by quantitative competitive RT-PCR. / Pinti, Marcello; Pedrazzi, Jessica; Benatti, Francesca; Sorrentino, Veronica; Nuzzo, Cira; Cavazzuti, Valeria; Biswas, Priscilla; Petrusca, Daniela; Mussini, Cristina; De Rienzo, Bruno; Cossarizza, Andrea.

In: FEBS Letters, Vol. 458, No. 2, 17.09.1999, p. 209-214.

Research output: Contribution to journalArticle

Pinti, Marcello ; Pedrazzi, Jessica ; Benatti, Francesca ; Sorrentino, Veronica ; Nuzzo, Cira ; Cavazzuti, Valeria ; Biswas, Priscilla ; Petrusca, Daniela ; Mussini, Cristina ; De Rienzo, Bruno ; Cossarizza, Andrea. / Differential down-regulation of CD95 or CD95L in chronically HIV-infected cells of monocytic or lymphocytic origin : Cellular studies and molecular analysis by quantitative competitive RT-PCR. In: FEBS Letters. 1999 ; Vol. 458, No. 2. pp. 209-214.
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AU - Sorrentino, Veronica

AU - Nuzzo, Cira

AU - Cavazzuti, Valeria

AU - Biswas, Priscilla

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