Differential effects of amino acid substitutions in the β-sheet floor and α-2 helix of HLA-A2 on recognition by alloreactive viral peptide-specific cytotoxic T lymphocytes

D. H. Mattson, N. Shimojo, E. P. Cowan, J. J. Baskin, R. V. Turner, B. D. Shvetsky, J. E. Coligan, W. L. Maloy, W. E. Biddison

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Abstract

Crystallographic studies of the HLA-A2 molecule have led to the assignment of a putative peptide binding site that consists of a groove with a β-pleated sheet floor bordered by two α-helices. A CTL-defined variant of HLA-A2, termed HLA-A2.2F, differs from the common A2.1 molecule by three amino acids: a Leu to Trp substitution at position 156 in the α-2 helix, a Val to Leu substitution at position 95 in the β-sheet floor of the groove, and a Gln to Arg substitution at position 43 in a loop outside of the groove. Another HLA-A2 variant, termed CLA, has a single Phe to Tyr substitution at position 9 that is sterically located adjacent to position 95 in the β-sheet floor of the groove. We have determined which of the amino acid substitutions at positions 9, 43, 95, or 156 could individually affect recognition by panels of A2.1 allospecific and A2.1-restricted influenza viral matrix peptide-specific CTL lines, using a panel of site-directed mutants and CLA. Recognition by allospecific CTL lines was generally unaffected by any one of the amino acid substitutions, but was eliminated by the double substitution at positions 95 and 156. Allorecognition by some CTL lines was eliminated by a single substitution at position 9 or 95. In contrast, recognition by A2.1-restricted matrix peptide specific CTL was totally eliminated by a single substitution at position 9 or 156. The substitution at position 43 in a loop away from the peptide binding groove had no effect on allorecognition or matrix peptide recognition. These results indicated that amino acid residues in the floor or α-2 helical wall of the peptide binding groove of the HLA-A2 molecule can differentially affect allorecognition and viral peptide recognition.

Original languageEnglish (US)
Pages (from-to)1101-1107
Number of pages7
JournalJournal of Immunology
Volume143
Issue number4
StatePublished - Jan 1 1989

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ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Mattson, D. H., Shimojo, N., Cowan, E. P., Baskin, J. J., Turner, R. V., Shvetsky, B. D., Coligan, J. E., Maloy, W. L., & Biddison, W. E. (1989). Differential effects of amino acid substitutions in the β-sheet floor and α-2 helix of HLA-A2 on recognition by alloreactive viral peptide-specific cytotoxic T lymphocytes. Journal of Immunology, 143(4), 1101-1107.