Differential effects of facial nerve transection on heat shock protein 70 expression in the developing and adult hamster facial nucleus

Geraldine A. Newfry, Kathryn Jones

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

In this study, the effects of axotomy on heat shock protein 70 (hsp70) protein levels were analyzed by immunoblotting with a hsp70 antibody that recognized constitutive and inducible forms of the protein. The right facial nerve of postnatal day 4 (neonate) or 100 (adult) hamsters was transected at its exit from the stylomastoid foramen, with the left nerve serving as internal control. Postoperative survival times were 2, 6, 12, and 24 h, with 3 animals per time point. Tissue punches containing individual facial nuclear groups were collected and homogenized. Approximately 10 μg of total protein was loaded onto paired gels and electrophoretically separated. Immunoblots of one gel from each pair were prepared, with the other gel stained in 0.2% Coomassie blue and used for verification of equality of lane loading. The results indicate that hsp70 protein levels increase in the adult, but decrease in the neonate, after axotomy. It is concluded that a stress response to cellular damage is an initial component of the classically described 'axon reaction' and that hsp70 plays a role in mediating motoneuron survival after peripheral nerve transection.

Original languageEnglish (US)
Pages (from-to)253-257
Number of pages5
JournalMetabolic Brain Disease
Volume13
Issue number3
DOIs
StatePublished - 1998
Externally publishedYes

Fingerprint

HSP70 Heat-Shock Proteins
Facial Nerve
Cricetinae
Axotomy
Gels
Proteins
Retrograde Degeneration
Motor Neurons
Peripheral Nerves
Immunoblotting
Animals
Tissue
Facial Nucleus
Antibodies

Keywords

  • Axotomy
  • Heat shock response
  • Nerve regeneration
  • Stress response

ASJC Scopus subject areas

  • Clinical Neurology
  • Biochemistry
  • Neuroscience(all)

Cite this

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title = "Differential effects of facial nerve transection on heat shock protein 70 expression in the developing and adult hamster facial nucleus",
abstract = "In this study, the effects of axotomy on heat shock protein 70 (hsp70) protein levels were analyzed by immunoblotting with a hsp70 antibody that recognized constitutive and inducible forms of the protein. The right facial nerve of postnatal day 4 (neonate) or 100 (adult) hamsters was transected at its exit from the stylomastoid foramen, with the left nerve serving as internal control. Postoperative survival times were 2, 6, 12, and 24 h, with 3 animals per time point. Tissue punches containing individual facial nuclear groups were collected and homogenized. Approximately 10 μg of total protein was loaded onto paired gels and electrophoretically separated. Immunoblots of one gel from each pair were prepared, with the other gel stained in 0.2{\%} Coomassie blue and used for verification of equality of lane loading. The results indicate that hsp70 protein levels increase in the adult, but decrease in the neonate, after axotomy. It is concluded that a stress response to cellular damage is an initial component of the classically described 'axon reaction' and that hsp70 plays a role in mediating motoneuron survival after peripheral nerve transection.",
keywords = "Axotomy, Heat shock response, Nerve regeneration, Stress response",
author = "Newfry, {Geraldine A.} and Kathryn Jones",
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AU - Jones, Kathryn

PY - 1998

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N2 - In this study, the effects of axotomy on heat shock protein 70 (hsp70) protein levels were analyzed by immunoblotting with a hsp70 antibody that recognized constitutive and inducible forms of the protein. The right facial nerve of postnatal day 4 (neonate) or 100 (adult) hamsters was transected at its exit from the stylomastoid foramen, with the left nerve serving as internal control. Postoperative survival times were 2, 6, 12, and 24 h, with 3 animals per time point. Tissue punches containing individual facial nuclear groups were collected and homogenized. Approximately 10 μg of total protein was loaded onto paired gels and electrophoretically separated. Immunoblots of one gel from each pair were prepared, with the other gel stained in 0.2% Coomassie blue and used for verification of equality of lane loading. The results indicate that hsp70 protein levels increase in the adult, but decrease in the neonate, after axotomy. It is concluded that a stress response to cellular damage is an initial component of the classically described 'axon reaction' and that hsp70 plays a role in mediating motoneuron survival after peripheral nerve transection.

AB - In this study, the effects of axotomy on heat shock protein 70 (hsp70) protein levels were analyzed by immunoblotting with a hsp70 antibody that recognized constitutive and inducible forms of the protein. The right facial nerve of postnatal day 4 (neonate) or 100 (adult) hamsters was transected at its exit from the stylomastoid foramen, with the left nerve serving as internal control. Postoperative survival times were 2, 6, 12, and 24 h, with 3 animals per time point. Tissue punches containing individual facial nuclear groups were collected and homogenized. Approximately 10 μg of total protein was loaded onto paired gels and electrophoretically separated. Immunoblots of one gel from each pair were prepared, with the other gel stained in 0.2% Coomassie blue and used for verification of equality of lane loading. The results indicate that hsp70 protein levels increase in the adult, but decrease in the neonate, after axotomy. It is concluded that a stress response to cellular damage is an initial component of the classically described 'axon reaction' and that hsp70 plays a role in mediating motoneuron survival after peripheral nerve transection.

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