Differential expression of cytosolic proteins in the rat kidney cortex and medulla

Preliminary proteomics

Frank Witzmann, Carla D. Fultz, Raymond A. Grant, Lynda S. Wright, Steven E. Kornguth, Frank L. Siegel

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

The rodent kidney is a target of many xenobiotics and is typified by regionally specific structure and function. This renders distinct regions of the kidney differentially susceptible to toxic exposure and effect. To characterize these differences at the proteome level, protein patterns from male rat kidney cortex and medulla cytosols were examined by two-dimensional electrophoresis (2-DE) and image analysis and prominent proteins identified immunologically or by matrix-assisted laser desorption/ionization - mass spectrometry (MALDI-MS) and electrospray/ionization - tandem mass spectrometry (ESI-MS/MS) sequence tag identification. An average of 727 protein spots were resolved and matched to the cortex cytosol reference pattern, and 716 in the medulla. Of this total, 127 proteins were found to differ in abundance (86 higher in cortex; 41 higher in medulla) (P < 0.001). Of those proteins that were detectable in both cortex and medulla, the abundance of 97 differed significantly while 30 proteins were found to be unique to one region or the other (26 in cortex, 4 in medulla). Twenty protein spots were identified and their regional differences are discussed. These results both confirm and expand our understanding of the molecular heterogeneity characterizing structurally and functionally distinct regions of the kidney and serve as a useful foundation for future nephrotoxicologic studies.

Original languageEnglish
Pages (from-to)2491-2497
Number of pages7
JournalElectrophoresis
Volume19
Issue number14
DOIs
StatePublished - Oct 1998

Fingerprint

Kidney Medulla
Kidney Cortex
Proteomics
Rats
Proteins
Kidney
Cytosol
Mass spectrometry
Electrospray ionization
Electrospray Ionization Mass Spectrometry
Poisons
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Xenobiotics
Proteome
Tandem Mass Spectrometry
Electrophoresis
Image analysis
Ionization
Rodentia
Desorption

Keywords

  • Cortex
  • Cytoplasm
  • Kidney
  • Medulla
  • Proteomics
  • Rat
  • Two-dimensional polyacrylamide gel electrophoresis

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Differential expression of cytosolic proteins in the rat kidney cortex and medulla : Preliminary proteomics. / Witzmann, Frank; Fultz, Carla D.; Grant, Raymond A.; Wright, Lynda S.; Kornguth, Steven E.; Siegel, Frank L.

In: Electrophoresis, Vol. 19, No. 14, 10.1998, p. 2491-2497.

Research output: Contribution to journalArticle

Witzmann, Frank ; Fultz, Carla D. ; Grant, Raymond A. ; Wright, Lynda S. ; Kornguth, Steven E. ; Siegel, Frank L. / Differential expression of cytosolic proteins in the rat kidney cortex and medulla : Preliminary proteomics. In: Electrophoresis. 1998 ; Vol. 19, No. 14. pp. 2491-2497.
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