Differential Expression of GRK Isoforms in Nonmalignant and Malignant Human Granulosa Cells

Denise Walker King, Rosemary Steinmetz, Heather A. Wagoner, Tamara Hannon, Lin Yuan Chen, Erica Eugster, Ora Hirsch Pescovitz

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Granulosa cell tumors are serious ovarian neoplasms that can occur in women of all ages. While there have been numerous attempts to understand the cause of these malignancies, the pathogenesis of granulosa cell tumors (GCTs) still remains largely unknown. G-protein coupled receptor kinases (GRKs) are important regulators of signal transduction through the process of receptor desensitization and internalization. Receptors that are regulated by GRKs are members of the large family of seven-transmembrane receptors and include the follicle stimulating hormone receptor (FSHR). In granulosa cells, the FSH signaling system is responsible for cell proliferation, differentiation, and steroidogenesis. In the studies presented, we examined GRK mRNA and protein expression in nonmalignant human granulosa cells, in KGN cells, a human GCT cell line, and in a panel of human GCT samples. The KGN tumor cells express significantly less GRK4 α/β protein and higher levels of GRK2 and GRK4 γ/δ protein as compared to nonmalignant human granulosa cells. In human GCT samples, GRK4 α/β protein was detected in 3 of the 13 tumor samples, whereas γ/δ proteins expression was detected in all samples. These findings suggest that GRK protein expression is altered in GCTs and may be involved in the pathogenesis of these tumors.

Original languageEnglish
Pages (from-to)135-141
Number of pages7
JournalEndocrine
Volume22
Issue number2
DOIs
StatePublished - Nov 2003

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G-Protein-Coupled Receptor Kinases
Granulosa Cell Tumor
Granulosa Cells
Protein Isoforms
Proteins
Neoplasms
FSH Receptors
Tumor Cell Line
Ovarian Neoplasms
Cell Differentiation
Signal Transduction
Cell Proliferation
Messenger RNA

Keywords

  • Cell signaling
  • Granulosa cell tumors
  • GRKs
  • Ovary

ASJC Scopus subject areas

  • Endocrinology

Cite this

Differential Expression of GRK Isoforms in Nonmalignant and Malignant Human Granulosa Cells. / King, Denise Walker; Steinmetz, Rosemary; Wagoner, Heather A.; Hannon, Tamara; Chen, Lin Yuan; Eugster, Erica; Pescovitz, Ora Hirsch.

In: Endocrine, Vol. 22, No. 2, 11.2003, p. 135-141.

Research output: Contribution to journalArticle

King, Denise Walker ; Steinmetz, Rosemary ; Wagoner, Heather A. ; Hannon, Tamara ; Chen, Lin Yuan ; Eugster, Erica ; Pescovitz, Ora Hirsch. / Differential Expression of GRK Isoforms in Nonmalignant and Malignant Human Granulosa Cells. In: Endocrine. 2003 ; Vol. 22, No. 2. pp. 135-141.
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