Differential expression of N-myc in phenotypically distinct subclones of a human neuroblastoma cell line

John Foley, Susan L. Cohn, Helen R. Salwen, Daniel Chagnovich, Janet Cowan, Karen L. Mason, Linda M. Parysek

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

Neuroblastomas are malignant childhood neoplasms that arise from derivatives of the neural crest. We report the characterization of a new neuroblastoma cell line, designated NBL-W, derived from the primary tumor of a patient with stage IVS disease (S. L. Cohn, C. V. Herst, H. S. Maurer, and S. T. Rosen, J. Clin. Oncol., 5: 1441-1444, 1987) according to the criteria of Evans [A. E. Evans, G. J. D'Angio, and J. Randolf, Cancer (Phila.), 27: 374-378, 1971]. Neurite-bearing (N) and substrate-adherent (S) cell lines have been subcloned from the parent line. N and S cells can interconvert, and both cell types label with the neural crest cell surface marker antibody, HNK-1. Cells in the subcloned lines and in the parent line have been shown by Southern blot analysis to contain ̈100 copies of the N-myc gene. Cytogenetic analysis shows a homogeneously staining region present on chromosome 19. Although these subclones are of identical genotype, the S cells express lower amounts of N-myc mRNA and protein as compared to the N cells. N cells express several neuronal proteins including the neurotransmitter-processing enzymes tyrosine hydroxylase and dopamine β-hydroxylase, the neuronal intermediate filament proteins peripherin and NF66/α-internexin, and the neural cell adhesion molecule. S cells generally lack neuronal markers but express the mesenchymal intermediate filament protein vimentin, and a small subset of the S cells express glial fibrillary acidic protein. Some S cells were labeled weakly with neural cell adhesion molecule antibody; others were negative. S cells did not express the glial marker S-100 or a melanocyte marker, tyrosinase. Thus, S cells express the neural crest marker HNK-1 but do not express a set of antigens characteristic of any known cell type derived from the neural crest. These results are consistent with the suggestion that differential N-myc expression may be involved in the interconversion of N and S cells but indicate that the S cell phenotype need not represent a highly differentiated neural crest derivative.

Original languageEnglish (US)
Pages (from-to)6338-6345
Number of pages8
JournalCancer Research
Volume51
Issue number23
StatePublished - Dec 1 1991
Externally publishedYes

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Neuroblastoma
Cell Line
Neural Crest
Intermediate Filament Proteins
Neural Cell Adhesion Molecules
Peripherins
Chromosomes, Human, Pair 19
Neoplasms
myc Genes
Monophenol Monooxygenase
Antibodies
Cytogenetic Analysis
Melanocytes
Glial Fibrillary Acidic Protein
Tyrosine 3-Monooxygenase
Vimentin
Neurites
Southern Blotting
Mixed Function Oxygenases
Neuroglia

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Foley, J., Cohn, S. L., Salwen, H. R., Chagnovich, D., Cowan, J., Mason, K. L., & Parysek, L. M. (1991). Differential expression of N-myc in phenotypically distinct subclones of a human neuroblastoma cell line. Cancer Research, 51(23), 6338-6345.

Differential expression of N-myc in phenotypically distinct subclones of a human neuroblastoma cell line. / Foley, John; Cohn, Susan L.; Salwen, Helen R.; Chagnovich, Daniel; Cowan, Janet; Mason, Karen L.; Parysek, Linda M.

In: Cancer Research, Vol. 51, No. 23, 01.12.1991, p. 6338-6345.

Research output: Contribution to journalArticle

Foley, J, Cohn, SL, Salwen, HR, Chagnovich, D, Cowan, J, Mason, KL & Parysek, LM 1991, 'Differential expression of N-myc in phenotypically distinct subclones of a human neuroblastoma cell line', Cancer Research, vol. 51, no. 23, pp. 6338-6345.
Foley J, Cohn SL, Salwen HR, Chagnovich D, Cowan J, Mason KL et al. Differential expression of N-myc in phenotypically distinct subclones of a human neuroblastoma cell line. Cancer Research. 1991 Dec 1;51(23):6338-6345.
Foley, John ; Cohn, Susan L. ; Salwen, Helen R. ; Chagnovich, Daniel ; Cowan, Janet ; Mason, Karen L. ; Parysek, Linda M. / Differential expression of N-myc in phenotypically distinct subclones of a human neuroblastoma cell line. In: Cancer Research. 1991 ; Vol. 51, No. 23. pp. 6338-6345.
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abstract = "Neuroblastomas are malignant childhood neoplasms that arise from derivatives of the neural crest. We report the characterization of a new neuroblastoma cell line, designated NBL-W, derived from the primary tumor of a patient with stage IVS disease (S. L. Cohn, C. V. Herst, H. S. Maurer, and S. T. Rosen, J. Clin. Oncol., 5: 1441-1444, 1987) according to the criteria of Evans [A. E. Evans, G. J. D'Angio, and J. Randolf, Cancer (Phila.), 27: 374-378, 1971]. Neurite-bearing (N) and substrate-adherent (S) cell lines have been subcloned from the parent line. N and S cells can interconvert, and both cell types label with the neural crest cell surface marker antibody, HNK-1. Cells in the subcloned lines and in the parent line have been shown by Southern blot analysis to contain ̈100 copies of the N-myc gene. Cytogenetic analysis shows a homogeneously staining region present on chromosome 19. Although these subclones are of identical genotype, the S cells express lower amounts of N-myc mRNA and protein as compared to the N cells. N cells express several neuronal proteins including the neurotransmitter-processing enzymes tyrosine hydroxylase and dopamine β-hydroxylase, the neuronal intermediate filament proteins peripherin and NF66/α-internexin, and the neural cell adhesion molecule. S cells generally lack neuronal markers but express the mesenchymal intermediate filament protein vimentin, and a small subset of the S cells express glial fibrillary acidic protein. Some S cells were labeled weakly with neural cell adhesion molecule antibody; others were negative. S cells did not express the glial marker S-100 or a melanocyte marker, tyrosinase. Thus, S cells express the neural crest marker HNK-1 but do not express a set of antigens characteristic of any known cell type derived from the neural crest. These results are consistent with the suggestion that differential N-myc expression may be involved in the interconversion of N and S cells but indicate that the S cell phenotype need not represent a highly differentiated neural crest derivative.",
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N2 - Neuroblastomas are malignant childhood neoplasms that arise from derivatives of the neural crest. We report the characterization of a new neuroblastoma cell line, designated NBL-W, derived from the primary tumor of a patient with stage IVS disease (S. L. Cohn, C. V. Herst, H. S. Maurer, and S. T. Rosen, J. Clin. Oncol., 5: 1441-1444, 1987) according to the criteria of Evans [A. E. Evans, G. J. D'Angio, and J. Randolf, Cancer (Phila.), 27: 374-378, 1971]. Neurite-bearing (N) and substrate-adherent (S) cell lines have been subcloned from the parent line. N and S cells can interconvert, and both cell types label with the neural crest cell surface marker antibody, HNK-1. Cells in the subcloned lines and in the parent line have been shown by Southern blot analysis to contain ̈100 copies of the N-myc gene. Cytogenetic analysis shows a homogeneously staining region present on chromosome 19. Although these subclones are of identical genotype, the S cells express lower amounts of N-myc mRNA and protein as compared to the N cells. N cells express several neuronal proteins including the neurotransmitter-processing enzymes tyrosine hydroxylase and dopamine β-hydroxylase, the neuronal intermediate filament proteins peripherin and NF66/α-internexin, and the neural cell adhesion molecule. S cells generally lack neuronal markers but express the mesenchymal intermediate filament protein vimentin, and a small subset of the S cells express glial fibrillary acidic protein. Some S cells were labeled weakly with neural cell adhesion molecule antibody; others were negative. S cells did not express the glial marker S-100 or a melanocyte marker, tyrosinase. Thus, S cells express the neural crest marker HNK-1 but do not express a set of antigens characteristic of any known cell type derived from the neural crest. These results are consistent with the suggestion that differential N-myc expression may be involved in the interconversion of N and S cells but indicate that the S cell phenotype need not represent a highly differentiated neural crest derivative.

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