Differential gene expression of neonatal and adult DRG neurons correlates with the differential sensitization of TRPV1 responses to nerve growth factor

Weiguo Zhu, Gerry S. Oxford

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Cultures of neonatal and adult dorsal root ganglion (DRG) neurons are commonly used in in vitro models to study the ion channels and signaling events associated with peripheral sensation under various conditions. Differential responsiveness between neonatal and adult DRG neurons to physiological or pathological stimuli suggests potential differences in their gene expression profiles. We performed a microarray analysis of cultured adult and neonatal rat DRG neurons, which revealed distinct gene expression profiles especially of ion channels and signaling molecules at the genomic level. For example, Ca2+-stimulated adenylyl cyclase (AC) isoforms AC3 and AC8, PKCδ and CaMKIIα, the voltage-gated sodium channel β1 and β4, and potassium channels Kv1.1, Kv3.2, Kv4.1, Kv9.1, Kv9.3, Kir3.4, Kir7.1, K2P1.1/TWIK-1 had significantly higher mRNA expression in adult rat DRG neurons, while Ca2+-inhibited AC5 and AC6, sodium channel Nav1.3 α subunit, potassium channels Kir6.1, K2P10.1/TREK-2, calcium channel Cav2.2 α1 subunit, and its auxiliary subunits β1 and β3 were conversely down regulated in adult neurons. Importantly, higher adult neuron expression of ERK1/2, PI3K/P110α, but not of TRPV1 and TrkA, was found and confirmed by PCR and western blot. These latter findings are consistent with the key role of ERK and PI3K signaling in sensitization of TRPV1 by NGF and may explain our previously published observation that adult, but not neonatal, rat DRG neurons are sensitized by NGF.

Original languageEnglish
Pages (from-to)192-196
Number of pages5
JournalNeuroscience Letters
Volume500
Issue number3
DOIs
StatePublished - Aug 18 2011

Fingerprint

Spinal Ganglia
Nerve Growth Factor
Gene Expression
Neurons
Phosphatidylinositol 3-Kinases
Ion Channels
Transcriptome
Shaw Potassium Channels
Kv1.1 Potassium Channel
Voltage-Gated Sodium Channels
Calcium-Calmodulin-Dependent Protein Kinase Type 2
Sodium Channels
Potassium Channels
Calcium Channels
Microarray Analysis
Adenylyl Cyclases
Protein Isoforms
Western Blotting
Observation
Polymerase Chain Reaction

Keywords

  • Dorsal root ganglion
  • ERK1/2
  • Ion channels
  • Microarray
  • PI3K/P110α

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Differential gene expression of neonatal and adult DRG neurons correlates with the differential sensitization of TRPV1 responses to nerve growth factor. / Zhu, Weiguo; Oxford, Gerry S.

In: Neuroscience Letters, Vol. 500, No. 3, 18.08.2011, p. 192-196.

Research output: Contribution to journalArticle

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