Differential influence of the 4F2 heavy chain and the protein related to b0,+ amino acid transport on substrate affinity of the heteromeric b0,+ amino acid transporter

D. Prasanna Rajan, Wei Huang, Ramesh Kekuda, Ronald L. George, Jian Wang, Simon J. Conway, Lawrence D. Devoe, Frederick H. Leibach, Puttur D. Prasad, Vadivel Ganapathy

Research output: Contribution to journalArticle

44 Scopus citations

Abstract

We provide evidence here that b0,+ amino acid transporter (b0,+ AT) interacts with 4F2 heavy chain (4F2hc) as well as with the protein related to b amino acid transporter (rBAT) to constitute functionally competent b0,+-like amino acid transport systems. This evidence has been obtained by co-expression of b0,+ AT and 4F2hc or b0,+AT and rBAT in human retinal pigment epithelial cells and in COS-1 cells. The ability to interact with 4F2hc and rBAT is demonstrable with mouse b0,+AT as well as with human b0,+AT. Even though both the 4F2hc.b0,+AT complex and the rBAT·b0,+ AT complex exhibit substrate specificity that is characteristic of system b0,+, these two complexes differ significantly in substrate affinity. The 4F2hc·b0,+AT complex has higher substrate affinity than the rBAT·b0,+AT complex. In situ hybridization studies demonstrate that the regional distribution pattern of mRNA in the kidney is identical for b0,+ AT and 4F2hc. The pattern of rBAT mRNA expression is different from that of b0,+AT mRNA and 4F2hc mRNA, but there are regions in the kidney where b0,+AT mRNA expression overlaps with rBAT mRNA expression as well as with 4F2hc mRNA expression.

Original languageEnglish (US)
Pages (from-to)14331-14335
Number of pages5
JournalJournal of Biological Chemistry
Volume275
Issue number19
DOIs
StatePublished - May 12 2000

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Differential influence of the 4F2 heavy chain and the protein related to b<sup>0,+</sup> amino acid transport on substrate affinity of the heteromeric b<sup>0,+</sup> amino acid transporter'. Together they form a unique fingerprint.

  • Cite this