Differential influence of the 4F2 heavy chain and the protein related to b0,+ amino acid transport on substrate affinity of the heteromeric b0,+ amino acid transporter

D. Prasanna Rajan, Wei Huang, Ramesh Kekuda, Ronald L. George, Jian Wang, Simon Conway, Lawrence D. Devoe, Frederick H. Leibach, Puttur D. Prasad, Vadivel Ganapathy

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Abstract

We provide evidence here that b0,+ amino acid transporter (b0,+ AT) interacts with 4F2 heavy chain (4F2hc) as well as with the protein related to b amino acid transporter (rBAT) to constitute functionally competent b0,+-like amino acid transport systems. This evidence has been obtained by co-expression of b0,+ AT and 4F2hc or b0,+AT and rBAT in human retinal pigment epithelial cells and in COS-1 cells. The ability to interact with 4F2hc and rBAT is demonstrable with mouse b0,+AT as well as with human b0,+AT. Even though both the 4F2hc.b0,+AT complex and the rBAT·b0,+ AT complex exhibit substrate specificity that is characteristic of system b0,+, these two complexes differ significantly in substrate affinity. The 4F2hc·b0,+AT complex has higher substrate affinity than the rBAT·b0,+AT complex. In situ hybridization studies demonstrate that the regional distribution pattern of mRNA in the kidney is identical for b0,+ AT and 4F2hc. The pattern of rBAT mRNA expression is different from that of b0,+AT mRNA and 4F2hc mRNA, but there are regions in the kidney where b0,+AT mRNA expression overlaps with rBAT mRNA expression as well as with 4F2hc mRNA expression.

Original languageEnglish (US)
Pages (from-to)14331-14335
Number of pages5
JournalJournal of Biological Chemistry
Volume275
Issue number19
DOIs
StatePublished - May 12 2000
Externally publishedYes

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CD98 Heavy Chain Antigens
Amino Acid Transport Systems
Amino Acids
Substrates
Messenger RNA
Proteins
Kidney
Retinal Pigments
COS Cells
Substrate Specificity
In Situ Hybridization

ASJC Scopus subject areas

  • Biochemistry

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Differential influence of the 4F2 heavy chain and the protein related to b0,+ amino acid transport on substrate affinity of the heteromeric b0,+ amino acid transporter. / Rajan, D. Prasanna; Huang, Wei; Kekuda, Ramesh; George, Ronald L.; Wang, Jian; Conway, Simon; Devoe, Lawrence D.; Leibach, Frederick H.; Prasad, Puttur D.; Ganapathy, Vadivel.

In: Journal of Biological Chemistry, Vol. 275, No. 19, 12.05.2000, p. 14331-14335.

Research output: Contribution to journalArticle

Rajan, D. Prasanna ; Huang, Wei ; Kekuda, Ramesh ; George, Ronald L. ; Wang, Jian ; Conway, Simon ; Devoe, Lawrence D. ; Leibach, Frederick H. ; Prasad, Puttur D. ; Ganapathy, Vadivel. / Differential influence of the 4F2 heavy chain and the protein related to b0,+ amino acid transport on substrate affinity of the heteromeric b0,+ amino acid transporter. In: Journal of Biological Chemistry. 2000 ; Vol. 275, No. 19. pp. 14331-14335.
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