Differential localization of transforming growth factor-β isoforms in human gastric mucosa and overexpression in gastric carcinoma

Markus Naef, Toshiyuki Ishiwata, Helmut Friess, Markus W. Büchler, Leslie I. Gold, Murray Korc

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Transforming growth factor β (TGF-β) isoforms comprise a family of multifunctional polypeptide growth factors that either inhibit or stimulate cell proliferation. We examined TGF-β expression in normal human gastric mucosa and carcinoma. The distribution and expression of TGF-β isoforms in 4 normal mucosa samples from organ donors, in 12 normal mucosa samples adjacent to gastric cancer and in 12 gastric carcinomas were examined using immunohistochemistry and Northern blot analysis. Because TGF-βs regulate collagen expression, collagen type I α1 mRNA amounts were also examined. Immunohistochemical analysis of normal human gastric tissue samples indicated that TGF-β1 localized principally in parietal cells but also in some surface mucus cells, TGF-β2 was present exclusively in chief cells and TGF- β3 was present in parietal, chief and mucus cells. In the gastric cancers, strong colocalization of TGF-β1, -β2 and -β3 was evident in the cancer cells. Northern blot analysis indicated that, compared to normal gastric tissue, gastric cancers showed a 4.8- and 6-fold increase in mRNA amounts encoding TGF-β1 and TGF-β3, respectively. In contrast, TGF-β2 mRNA amounts were comparable in both groups. Northern blot analysis showed a 10- fold increase in human collagen type I α1 mRNA amounts compared to normal gastric tissue. These findings imply a role for TGF-βs in normal human gastric mucosa function, and raise the possibility that the aberrant colocalization and overexpression of all 3 TGF-β isoforms in human gastric cancer cells in vivo may contribute to the pathobiology of gastric carcinoma.

Original languageEnglish (US)
Pages (from-to)131-137
Number of pages7
JournalInternational Journal of Cancer
Volume71
Issue number2
DOIs
StatePublished - 1997
Externally publishedYes

Fingerprint

Transforming Growth Factors
Gastric Mucosa
Stomach
Protein Isoforms
Carcinoma
Stomach Neoplasms
Northern Blotting
Messenger RNA
Mucus
Collagen Type I
Mucous Membrane
Intercellular Signaling Peptides and Proteins
Collagen
Immunohistochemistry
Cell Proliferation
Tissue Donors

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Differential localization of transforming growth factor-β isoforms in human gastric mucosa and overexpression in gastric carcinoma. / Naef, Markus; Ishiwata, Toshiyuki; Friess, Helmut; Büchler, Markus W.; Gold, Leslie I.; Korc, Murray.

In: International Journal of Cancer, Vol. 71, No. 2, 1997, p. 131-137.

Research output: Contribution to journalArticle

Naef, Markus ; Ishiwata, Toshiyuki ; Friess, Helmut ; Büchler, Markus W. ; Gold, Leslie I. ; Korc, Murray. / Differential localization of transforming growth factor-β isoforms in human gastric mucosa and overexpression in gastric carcinoma. In: International Journal of Cancer. 1997 ; Vol. 71, No. 2. pp. 131-137.
@article{03c36ee14f9d488eb9c0899340e82800,
title = "Differential localization of transforming growth factor-β isoforms in human gastric mucosa and overexpression in gastric carcinoma",
abstract = "Transforming growth factor β (TGF-β) isoforms comprise a family of multifunctional polypeptide growth factors that either inhibit or stimulate cell proliferation. We examined TGF-β expression in normal human gastric mucosa and carcinoma. The distribution and expression of TGF-β isoforms in 4 normal mucosa samples from organ donors, in 12 normal mucosa samples adjacent to gastric cancer and in 12 gastric carcinomas were examined using immunohistochemistry and Northern blot analysis. Because TGF-βs regulate collagen expression, collagen type I α1 mRNA amounts were also examined. Immunohistochemical analysis of normal human gastric tissue samples indicated that TGF-β1 localized principally in parietal cells but also in some surface mucus cells, TGF-β2 was present exclusively in chief cells and TGF- β3 was present in parietal, chief and mucus cells. In the gastric cancers, strong colocalization of TGF-β1, -β2 and -β3 was evident in the cancer cells. Northern blot analysis indicated that, compared to normal gastric tissue, gastric cancers showed a 4.8- and 6-fold increase in mRNA amounts encoding TGF-β1 and TGF-β3, respectively. In contrast, TGF-β2 mRNA amounts were comparable in both groups. Northern blot analysis showed a 10- fold increase in human collagen type I α1 mRNA amounts compared to normal gastric tissue. These findings imply a role for TGF-βs in normal human gastric mucosa function, and raise the possibility that the aberrant colocalization and overexpression of all 3 TGF-β isoforms in human gastric cancer cells in vivo may contribute to the pathobiology of gastric carcinoma.",
author = "Markus Naef and Toshiyuki Ishiwata and Helmut Friess and B{\"u}chler, {Markus W.} and Gold, {Leslie I.} and Murray Korc",
year = "1997",
doi = "10.1002/(SICI)1097-0215(19970410)71:2<131::AID-IJC1>3.0.CO;2-1",
language = "English (US)",
volume = "71",
pages = "131--137",
journal = "International Journal of Cancer",
issn = "0020-7136",
publisher = "Wiley-Liss Inc.",
number = "2",

}

TY - JOUR

T1 - Differential localization of transforming growth factor-β isoforms in human gastric mucosa and overexpression in gastric carcinoma

AU - Naef, Markus

AU - Ishiwata, Toshiyuki

AU - Friess, Helmut

AU - Büchler, Markus W.

AU - Gold, Leslie I.

AU - Korc, Murray

PY - 1997

Y1 - 1997

N2 - Transforming growth factor β (TGF-β) isoforms comprise a family of multifunctional polypeptide growth factors that either inhibit or stimulate cell proliferation. We examined TGF-β expression in normal human gastric mucosa and carcinoma. The distribution and expression of TGF-β isoforms in 4 normal mucosa samples from organ donors, in 12 normal mucosa samples adjacent to gastric cancer and in 12 gastric carcinomas were examined using immunohistochemistry and Northern blot analysis. Because TGF-βs regulate collagen expression, collagen type I α1 mRNA amounts were also examined. Immunohistochemical analysis of normal human gastric tissue samples indicated that TGF-β1 localized principally in parietal cells but also in some surface mucus cells, TGF-β2 was present exclusively in chief cells and TGF- β3 was present in parietal, chief and mucus cells. In the gastric cancers, strong colocalization of TGF-β1, -β2 and -β3 was evident in the cancer cells. Northern blot analysis indicated that, compared to normal gastric tissue, gastric cancers showed a 4.8- and 6-fold increase in mRNA amounts encoding TGF-β1 and TGF-β3, respectively. In contrast, TGF-β2 mRNA amounts were comparable in both groups. Northern blot analysis showed a 10- fold increase in human collagen type I α1 mRNA amounts compared to normal gastric tissue. These findings imply a role for TGF-βs in normal human gastric mucosa function, and raise the possibility that the aberrant colocalization and overexpression of all 3 TGF-β isoforms in human gastric cancer cells in vivo may contribute to the pathobiology of gastric carcinoma.

AB - Transforming growth factor β (TGF-β) isoforms comprise a family of multifunctional polypeptide growth factors that either inhibit or stimulate cell proliferation. We examined TGF-β expression in normal human gastric mucosa and carcinoma. The distribution and expression of TGF-β isoforms in 4 normal mucosa samples from organ donors, in 12 normal mucosa samples adjacent to gastric cancer and in 12 gastric carcinomas were examined using immunohistochemistry and Northern blot analysis. Because TGF-βs regulate collagen expression, collagen type I α1 mRNA amounts were also examined. Immunohistochemical analysis of normal human gastric tissue samples indicated that TGF-β1 localized principally in parietal cells but also in some surface mucus cells, TGF-β2 was present exclusively in chief cells and TGF- β3 was present in parietal, chief and mucus cells. In the gastric cancers, strong colocalization of TGF-β1, -β2 and -β3 was evident in the cancer cells. Northern blot analysis indicated that, compared to normal gastric tissue, gastric cancers showed a 4.8- and 6-fold increase in mRNA amounts encoding TGF-β1 and TGF-β3, respectively. In contrast, TGF-β2 mRNA amounts were comparable in both groups. Northern blot analysis showed a 10- fold increase in human collagen type I α1 mRNA amounts compared to normal gastric tissue. These findings imply a role for TGF-βs in normal human gastric mucosa function, and raise the possibility that the aberrant colocalization and overexpression of all 3 TGF-β isoforms in human gastric cancer cells in vivo may contribute to the pathobiology of gastric carcinoma.

UR - http://www.scopus.com/inward/record.url?scp=0031005355&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031005355&partnerID=8YFLogxK

U2 - 10.1002/(SICI)1097-0215(19970410)71:2<131::AID-IJC1>3.0.CO;2-1

DO - 10.1002/(SICI)1097-0215(19970410)71:2<131::AID-IJC1>3.0.CO;2-1

M3 - Article

VL - 71

SP - 131

EP - 137

JO - International Journal of Cancer

JF - International Journal of Cancer

SN - 0020-7136

IS - 2

ER -