Differential plasma clearance of murine acute-phase serum amyloid A proteins SAA1 and SAA2

Barbara Kluve-Beckerman, Toshiyuki Yamada, Joyce Hardwick, Juris J. Liepnieks, Merrill Benson

Research output: Contribution to journalArticle

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Abstract

Serum amyloid A (SAA) proteins SAA1 and SAA2 are prominent acute-phase reactants which circulate in association with the high-density-lipoprotein (HDL) fraction of plasma. Plasma levels of SAA1 and SAA2 increase dramatically, by as much as 1000-fold, within 24 h of tissue injury and then rapidly decrease with cessation of the inflammatory stimulus, suggesting that SAA clearance and/or catabolism is important to the re-establishment of homoeostasis. In this context, aberrant SAA catabolism has long been considered a potential factor in the pathogenesis of reactive amyloidosis. To initiate studies aimed at understanding the differential regulation of SAA metabolism, we have produced 35S-labelled murine SAA1 and SAA2 in Escherichia coli, bound them individually to HDL, and then compared the plasma-clearance characteristics of SAA1 and SAA2 under normal and acute-phase conditions. When bound to normal HDL, SAA2 [half-life(t( 1/4 )) = 30 min] was cleared significantly faster than SAA1 (t( 1/4 ) = 75 min). Clearance of SAA1 and SAA2 was significantly slower when each was bound to acute-phase HDL as opposed to normal HDL, when clearance rates were determined in acute-phase mice versus normal mice, and when normal HDL was remodelled to contain both recombinant isotypes rather than just one of the isotypes. Thus it appears that an increased amount of SAA on HDL, or possibly the combined presence of both isotypes on HDL, is associated with a prolongation in the plasma half-life of SAA.

Original languageEnglish
Pages (from-to)663-669
Number of pages7
JournalBiochemical Journal
Volume322
Issue number2
StatePublished - Mar 1 1997

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Serum Amyloid A Protein
HDL Lipoproteins
Plasmas
Half-Life
Acute-Phase Proteins
Amyloidosis
Metabolism
Escherichia coli
Homeostasis
Tissue
Wounds and Injuries

ASJC Scopus subject areas

  • Biochemistry

Cite this

Kluve-Beckerman, B., Yamada, T., Hardwick, J., Liepnieks, J. J., & Benson, M. (1997). Differential plasma clearance of murine acute-phase serum amyloid A proteins SAA1 and SAA2. Biochemical Journal, 322(2), 663-669.

Differential plasma clearance of murine acute-phase serum amyloid A proteins SAA1 and SAA2. / Kluve-Beckerman, Barbara; Yamada, Toshiyuki; Hardwick, Joyce; Liepnieks, Juris J.; Benson, Merrill.

In: Biochemical Journal, Vol. 322, No. 2, 01.03.1997, p. 663-669.

Research output: Contribution to journalArticle

Kluve-Beckerman, B, Yamada, T, Hardwick, J, Liepnieks, JJ & Benson, M 1997, 'Differential plasma clearance of murine acute-phase serum amyloid A proteins SAA1 and SAA2', Biochemical Journal, vol. 322, no. 2, pp. 663-669.
Kluve-Beckerman B, Yamada T, Hardwick J, Liepnieks JJ, Benson M. Differential plasma clearance of murine acute-phase serum amyloid A proteins SAA1 and SAA2. Biochemical Journal. 1997 Mar 1;322(2):663-669.
Kluve-Beckerman, Barbara ; Yamada, Toshiyuki ; Hardwick, Joyce ; Liepnieks, Juris J. ; Benson, Merrill. / Differential plasma clearance of murine acute-phase serum amyloid A proteins SAA1 and SAA2. In: Biochemical Journal. 1997 ; Vol. 322, No. 2. pp. 663-669.
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