Differential proliferative effects of transforming growth factor-β on human hematopoietic progenitor cells

O. G. Ottmann, Louis Pelus

Research output: Contribution to journalArticle

141 Citations (Scopus)

Abstract

Transforming growth factor-β (TGFβ) regulates cell growth and differentiation in numerous cell systems, including several hematopoietic lineages. We used in vitro cultures of highly enriched hematopoietic progenitor cells stimulated by natural and recombinant growth factors to investigate the biologic effects of TGFβ1 and TGFβ2 on erythroid (CFU-E and burst-forming unit (BFU)-E, granulocyte-macrophage (CFU-GM) and multilineage (i.e., granulocyte, erythroid, macrophage, and megakaryocyte; CFU-GEMM) colony-forming cells. In the absence of exogenous CSF, neither TGFβ1 nor TGFβ2 supported progenitor cell growth. In the presence of recombinant or natural CSF, picomolar concentrations of TGFβ1 inhibited growth of CFU-E, BFU-E and CFU-GEMM and enhanced growth of day 7 CFU-GM. Inhibition of CFU-E and BFU-E by human and porcine TGFβ1 was similar, ranging from 17 to 73% over a concentration range of 0.05 to 1.0 ng/ml, and was largely independent of the type of burst-promoting activity used (rIL-3 vs cell 5637-conditioned medium). Inhibition of CFU-GEMM ranged from 79 to 98% over a concentration range of 0.25 to 1.0 ng/ml. The inhibitory effect of TGFβ1 was progressively lost when its addition was delayed for 40 to 120 h, suggesting a mode of action during early cell divisions. In contrast, growth of CFU-GM stimulated by plateau concentrations of human rG-CSF, rGM-CSF, and rIL-3 was enhanced up to 154 ± 22% by human TGFβ1. Porcine platelet-derived TGFβ2 was essentially without effect on the progenitor populations examined. These results support the hypothesis that TGFβ may play a role in the regulation of hematopoietic progenitor cell proliferation by differentially affecting individual lineages and is apparently capable of doing so in the relative absence of marrow accessory cells.

Original languageEnglish (US)
Pages (from-to)2661-2665
Number of pages5
JournalJournal of Immunology
Volume140
Issue number8
StatePublished - 1988
Externally publishedYes

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Transforming Growth Factors
Hematopoietic Stem Cells
Myeloid Progenitor Cells
Granulocyte-Macrophage Progenitor Cells
Erythroid Precursor Cells
Growth
Granulocytes
Swine
Macrophages
Megakaryocytes
Conditioned Culture Medium
Cell Division
Cell Differentiation
Intercellular Signaling Peptides and Proteins
Stem Cells
Blood Platelets
Bone Marrow
Cell Proliferation
Population

ASJC Scopus subject areas

  • Immunology

Cite this

Differential proliferative effects of transforming growth factor-β on human hematopoietic progenitor cells. / Ottmann, O. G.; Pelus, Louis.

In: Journal of Immunology, Vol. 140, No. 8, 1988, p. 2661-2665.

Research output: Contribution to journalArticle

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