Differential protein expression following JP-8 jet fuel exposure

Frank Witzmann, Mark L. Witten

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

This chapter summarizes the collaborative efforts of two laboratories in assessing the toxicological effects of exposure to JP-8 jet fuel using several routes of exposure, and analyzing protein expression in various organ targets by proteomic techniques. JP-8 aerosol and vapor exposures included several rodent models where the effects on lung, liver, kidney, or testis tissues were evaluated. Additionally, in vitro exposures to JP-8 were conducted using alveolar type II epithelial cell cultures. While many insights into the effects of JP-8 exposure were gained through these studies, the limitations of the analytical approach also became self-evident. While two-dimensional gel electrophoresis (2-DE) is a powerful tool in the assessment of differential protein expression and is well-suited for toxicologic studies, the sensitivity of the approach clearly limited the scope of the analyses and many low-expression members of the target-tissue proteomes eluded clear interpretation. This statement is not to minimize the importance of the results, but rather to draw attention to recent developments in sample preparation for all manners of proteomic analyses, as described at the end of this chapter.

Original languageEnglish (US)
Title of host publicationJet Fuel Toxicology
PublisherCRC Press
Pages73-102
Number of pages30
ISBN (Electronic)9781420080216
ISBN (Print)9781420080209
DOIs
StatePublished - Jan 1 2010

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)
  • Medicine(all)
  • Environmental Science(all)

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  • Cite this

    Witzmann, F., & Witten, M. L. (2010). Differential protein expression following JP-8 jet fuel exposure. In Jet Fuel Toxicology (pp. 73-102). CRC Press. https://doi.org/10.1201/9781420080216