Differential regulation of cytoskeletal gene expression in hamster facial motoneurons

Effects of axotomy and testosterone treatment

Kathryn Jones, Paul D. Storer, Susan M. Drengler, Monica M. Oblinger

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

We have previously demonstrated that systemic administration of testosterone increases the rate of axonal regeneration following facial nerve crush in adult male hamsters. In the present study, the molecular mechanisms by which androgens could enhance axonal regeneration were examined at a cellular level. Specifically, the following question was addressed using quantitative in situ hybridization with cDNA probes complementary to βII, and α1 tubulin mRNAs: Does exogenous testosterone augment axotomy-induced changes in tubulin mRNA expression in hamster facial motoneurons (FMN)? Castrated adult male hamsters were subjected to right facial nerve severance, with the left side serving as internal control. One-half of the animals received testosterone replacement in the form of subcutaneously implanted silastic capsules containing crystalline testosterone propionate, and the other half were implanted with blank capsules immediately following the axotomy. Postoperative survival times from 2-14 days were examined. Axotomy alone resulted in a significant increase in the levels of both βII and α1 tubulin mRNAs in facial motor neurons between 2-14 days after injury. Administration of testosterone selectively augmented the axotomy-induced increases in βII-tubulin, but not α1 tubulin, mRNA, levels at 7 and 14 days post axotomy. These results demonstrating an effect of testosterone in altering the neuronal cytoskeletal response to axotomy suggest that testosterone may enhance the regenerative properties of motor neurons via molecular mechanisms that involve selective alterations of the neuronal cytoskeleton.

Original languageEnglish (US)
Pages (from-to)817-823
Number of pages7
JournalJournal of Neuroscience Research
Volume57
Issue number6
DOIs
StatePublished - Sep 15 1999
Externally publishedYes

Fingerprint

Axotomy
Gene Expression Regulation
Motor Neurons
Cricetinae
Testosterone
Tubulin
Messenger RNA
Facial Nerve
Capsules
Regeneration
Nerve Crush
Testosterone Propionate
Cytoskeleton
Androgens
In Situ Hybridization
Complementary DNA
Wounds and Injuries

Keywords

  • Axon injury
  • Gonadal steroids
  • Peripheral motor neurons
  • Regeneration
  • Tubulin mRNA

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Differential regulation of cytoskeletal gene expression in hamster facial motoneurons : Effects of axotomy and testosterone treatment. / Jones, Kathryn; Storer, Paul D.; Drengler, Susan M.; Oblinger, Monica M.

In: Journal of Neuroscience Research, Vol. 57, No. 6, 15.09.1999, p. 817-823.

Research output: Contribution to journalArticle

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