Differential repair of potentially lethal damage in exponentially growing and quiescent 9L cells

Marc Mendonca, A. Rodriguez, E. L. Alpen

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The alteration of potentially lethal damage repair by postirradiation treatment with hypertonic saline (0.5 m PBS) was investigated in exponentially growing and quiescent 9L cells in vitro. A single dose of X rays (8.5 Gy) immediately followed by a 30-min treatment with hypertonic PBS at 37°C reduced the survival of exponentially growing 9L cells by a factor of 13-18 compared to survival of irradiated immediately and delayed-plated cells, while the survival of quiescent cells was reduced by only a factor of 5-8. Survival curves confirmed the relative resistance of the quiescent 9L cells versus exponentially growing 9L cells to X rays plus hypertonic treatment. Both the slope and the shoulder of the survival curve were reduced to a greater extent in exponentially growing cells than in the quiescent cells by hypertonic treatment. The response of quiescent cells cannot be explained by either the duration of hypertonic treatment or the redistribution of the cells into G1 phase. We show that quiescent 9L cells can recover from hypertonically induced potentially lethal damage when incubated under conditions which have been found to delay progression through the cell cycle, and postulate that an altered chromatin structure or an enhanced repair capacity of quiescent 9L cells may be responsible for their resistance.

Original languageEnglish (US)
Pages (from-to)38-43
Number of pages6
JournalRadiation Research
Volume122
Issue number1
StatePublished - 1990
Externally publishedYes

Fingerprint

damage
cells
X-radiation
X-Rays
chromatin
G1 Phase
shoulders
curves
interphase
axioms
Chromatin
cell viability
progressions
cell cycle
Cell Survival
Cell Cycle
x rays
slopes
duration
dosage

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Radiology Nuclear Medicine and imaging
  • Biophysics
  • Radiation

Cite this

Differential repair of potentially lethal damage in exponentially growing and quiescent 9L cells. / Mendonca, Marc; Rodriguez, A.; Alpen, E. L.

In: Radiation Research, Vol. 122, No. 1, 1990, p. 38-43.

Research output: Contribution to journalArticle

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